Total antioxidant capacity of human blood plasma from healthy adults

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Reactive oxygen species are produced as a consequence of normal aerobic metabolism and could be important causative agents of a number of human diseases. Human serum contains many different antioxidants that may have importance of maintenance of antioxidant screen. Blood samples were obtained from healthy volunteers, at young (20–35 years) and old (36–60 years) age. Plasma was prepared and assayed within 3 h from blood collection and analyzed for the antioxidant capacity. At a low pH a ferric tripyridyltriazine (Fe3+-TPTZ) complex is reduced to the ferrous (Fe2+) form that causes changes of sample absorbance at 593 nm. The plasma antioxidant capacity of young and old group corresponded to 22.69 ± 0.86 and 17.59 ± 1.17 μmol of Trolox (a water-soluble analog of vitamin E). Young group had significantly higher ferric reducing/antioxidant power value than old subjects. These suggest that plasma antioxidant capacity declines with volunteers age.Acknowledgement:  The study was financed by a 502-11-809 Med. Univ. grant.

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