Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes

ISSN:
0165-1161
Keywords:
(Human) ; Flow cytometry ; HLA-A locus mutation ; Lymphocytes ; Somatic mutation
Source:
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
Topics:
Biology
Medicine
Type of Medium:
Electronic Resource
URL:
http://linkinghub.elsevier.com/retrieve/pii/0165-1161(92)90005-7
_version_ 1798291580067512322
autor Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
autorsonst Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
book_url http://linkinghub.elsevier.com/retrieve/pii/0165-1161(92)90005-7
datenlieferant nat_lic_papers
fussnote A flow-cytometric technique was developed to measure the frequency of variant lymphocytes lacking expression of HLA-A2 or A24 allele products among donors heterozygous for HLA-A2 or A24. It was found that the variant frequency of lymphocytes in peripheral blood was of the order of 10^-^4 and increased with donor age. Molecular analyses of mutant clones revealed that about one-third were derived from somatic recombinations and that the remaining two-thirds did not show any alterations after Southernblotting analysis. In contrast, mutants obtained after in vitro X-ray mutagenesis study were found to be mostly derived from large chromosomal deletions. A small-scale study on atomic bomb survivors did not show a significant dose effect.
hauptsatz hsatz_simple
identnr NLZ186685890
issn 0165-1161
journal_name Mutation Research/Environmental Mutagenesis and Related Subjects
materialart 1
package_name Elsevier
publikationsort Amsterdam
publisher Elsevier
reference 272 (1992), S. 17-29
schlagwort (Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
search_space articles
shingle_author_1 Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
shingle_author_2 Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
shingle_author_3 Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
shingle_author_4 Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
shingle_catch_all_1 Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
(Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
(Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
0165-1161
01651161
Elsevier
shingle_catch_all_2 Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
(Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
(Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
0165-1161
01651161
Elsevier
shingle_catch_all_3 Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
(Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
(Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
0165-1161
01651161
Elsevier
shingle_catch_all_4 Kushiro, J.-i.
Hirai, Y.
Kusunoki, Y.
Kyoizumi, S.
Kodama, Y.
Wakisaka, A.
Jeffreys, A.
Cologne, J.B.
Dohi, K.
Akiyama, M.
Nakamura, N.
Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
(Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
(Human)
Flow cytometry
HLA-A locus mutation
Lymphocytes
Somatic mutation
0165-1161
01651161
Elsevier
shingle_title_1 Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
shingle_title_2 Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
shingle_title_3 Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
shingle_title_4 Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
sigel_instance_filter dkfz
geomar
wilbert
ipn
albert
fhp
source_archive Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
timestamp 2024-05-06T08:34:53.093Z
titel Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
titel_suche Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes
A flow-cytometric technique was developed to measure the frequency of variant lymphocytes lacking expression of HLA-A2 or A24 allele products among donors heterozygous for HLA-A2 or A24. It was found that the variant frequency of lymphocytes in peripheral blood was of the order of 10^-^4 and increased with donor age. Molecular analyses of mutant clones revealed that about one-third were derived from somatic recombinations and that the remaining two-thirds did not show any alterations after Southernblotting analysis. In contrast, mutants obtained after in vitro X-ray mutagenesis study were found to be mostly derived from large chromosomal deletions. A small-scale study on atomic bomb survivors did not show a significant dose effect.
topic W
WW-YZ
uid nat_lic_papers_NLZ186685890