Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes - Kushiro, J.-i.; Hirai, Y.; Kusunoki, Y.; Kyoizumi, S.; Kodama, Y.; Wakisaka, A.; Jeffreys, A.; Cologne, J.B.; Dohi, K.; Akiyama, M.; Nakamura, N. (Elsevier)
A flow-cytometric technique was developed to measure the frequency of variant lymphocytes lacking expression of HLA-A2 or A24 allele products among donors heterozygous for HLA-A2 or A24. It was found that the variant frequency of lymphocytes in peripheral blood was of the order of 10^-^4 and increased with donor age. Molecular analyses of mutant clones revealed that about one-third were derived from somatic recombinations and that the remaining two-thirds did not show any alterations after Southernblotting analysis. In contrast, mutants obtained after in vitro X-ray mutagenesis study were found to be mostly derived from large chromosomal deletions. A small-scale study on atomic bomb survivors did not show a significant dose effect.
http://linkinghub.elsevier.com/retrieve/pii/0165-1161(92)90005-7
Keywords: W; WW-YZ