Determination of the degree of in vivo tRNA aminoacylation in yeast cells
| ISSN: |
0003-2697
|
|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292446184996866 |
|---|---|
| autor | Ehresmann, B. Imbault, P. Weil, J.H. |
| autorsonst | Ehresmann, B. Imbault, P. Weil, J.H. |
| book_url | http://dx.doi.org/10.1016/0003-2697(74)90423-0 |
| datenlieferant | nat_lic_papers |
| fussnote | A method is described to harvest yeast cells, extract tRNAs having a good biological activity, and measure the degree of in vivo tRNA aminoacylation. This measuremet is based on a determination of the percentage of tRNA which is resistant to periodate oxidation; control experiments have been performed to check that this treatment inactivates uncharged tRNA molecules, but does not affect aminoacylated ones, and allows therefore an accurate determination of the percentage of aminoacylated tRNA molecules. |
| hauptsatz | hsatz_simple |
| identnr | NLZ18404359X |
| issn | 0003-2697 |
| journal_name | Analytical Biochemistry |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 61 (1974), S. 548-556 |
| search_space | articles |
| shingle_author_1 | Ehresmann, B. Imbault, P. Weil, J.H. |
| shingle_author_2 | Ehresmann, B. Imbault, P. Weil, J.H. |
| shingle_author_3 | Ehresmann, B. Imbault, P. Weil, J.H. |
| shingle_author_4 | Ehresmann, B. Imbault, P. Weil, J.H. |
| shingle_catch_all_1 | Ehresmann, B. Imbault, P. Weil, J.H. Determination of the degree of in vivo tRNA aminoacylation in yeast cells 0003-2697 00032697 Elsevier |
| shingle_catch_all_2 | Ehresmann, B. Imbault, P. Weil, J.H. Determination of the degree of in vivo tRNA aminoacylation in yeast cells 0003-2697 00032697 Elsevier |
| shingle_catch_all_3 | Ehresmann, B. Imbault, P. Weil, J.H. Determination of the degree of in vivo tRNA aminoacylation in yeast cells 0003-2697 00032697 Elsevier |
| shingle_catch_all_4 | Ehresmann, B. Imbault, P. Weil, J.H. Determination of the degree of in vivo tRNA aminoacylation in yeast cells 0003-2697 00032697 Elsevier |
| shingle_title_1 | Determination of the degree of in vivo tRNA aminoacylation in yeast cells |
| shingle_title_2 | Determination of the degree of in vivo tRNA aminoacylation in yeast cells |
| shingle_title_3 | Determination of the degree of in vivo tRNA aminoacylation in yeast cells |
| shingle_title_4 | Determination of the degree of in vivo tRNA aminoacylation in yeast cells |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:48:38.883Z |
| titel | Determination of the degree of in vivo tRNA aminoacylation in yeast cells |
| titel_suche | Determination of the degree of in vivo tRNA aminoacylation in yeast cells A method is described to harvest yeast cells, extract tRNAs having a good biological activity, and measure the degree of in vivo tRNA aminoacylation. This measuremet is based on a determination of the percentage of tRNA which is resistant to periodate oxidation; control experiments have been performed to check that this treatment inactivates uncharged tRNA molecules, but does not affect aminoacylated ones, and allows therefore an accurate determination of the percentage of aminoacylated tRNA molecules. |
| topic | W V |
| uid | nat_lic_papers_NLZ18404359X |