Determination of the degree of in vivo tRNA aminoacylation in yeast cells - Ehresmann, B.; Imbault, P.; Weil, J.H. (Elsevier)
A method is described to harvest yeast cells, extract tRNAs having a good biological activity, and measure the degree of in vivo tRNA aminoacylation. This measuremet is based on a determination of the percentage of tRNA which is resistant to periodate oxidation; control experiments have been performed to check that this treatment inactivates uncharged tRNA molecules, but does not affect aminoacylated ones, and allows therefore an accurate determination of the percentage of aminoacylated tRNA molecules.
http://dx.doi.org/10.1016/0003-2697(74)90423-0
Keywords: W; V