Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness
Brookes, A. J. ; Slorach, E. M. ; Evans, K. L. ; Thomson, M. L. ; Gosden, C. M. ; Muir, W. J. ; Porteous, D. J.
Springer
Published 1995
Springer
Published 1995
| ISSN: |
1432-1777
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|---|---|
| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Medicine
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| Notes: |
Abstract An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798297661034463232 |
|---|---|
| autor | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. |
| autorsonst | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. |
| book_url | http://dx.doi.org/10.1007/BF00352412 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM207330360 |
| issn | 1432-1777 |
| journal_name | Mammalian genome |
| materialart | 1 |
| notes | Abstract An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction. |
| package_name | Springer |
| publikationsjahr_anzeige | 1995 |
| publikationsjahr_facette | 1995 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1995 |
| publisher | Springer |
| reference | 6 (1995), S. 257-262 |
| search_space | articles |
| shingle_author_1 | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. |
| shingle_author_2 | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. |
| shingle_author_3 | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. |
| shingle_author_4 | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. |
| shingle_catch_all_1 | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness Abstract An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction. 1432-1777 14321777 Springer |
| shingle_catch_all_2 | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness Abstract An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction. 1432-1777 14321777 Springer |
| shingle_catch_all_3 | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness Abstract An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction. 1432-1777 14321777 Springer |
| shingle_catch_all_4 | Brookes, A. J. Slorach, E. M. Evans, K. L. Thomson, M. L. Gosden, C. M. Muir, W. J. Porteous, D. J. Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness Abstract An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction. 1432-1777 14321777 Springer |
| shingle_title_1 | Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness |
| shingle_title_2 | Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness |
| shingle_title_3 | Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness |
| shingle_title_4 | Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T10:11:32.252Z |
| titel | Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness |
| titel_suche | Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLM207330360 |