Identifying genes within microdissected genomic DNA: Isolation of brain expressed genes from a translocation region associated with inherited mental illness
Brookes, A. J. ; Slorach, E. M. ; Evans, K. L. ; Thomson, M. L. ; Gosden, C. M. ; Muir, W. J. ; Porteous, D. J.
Springer
Published 1995
Springer
Published 1995
| ISSN: |
1432-1777
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|---|---|
| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Medicine
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| Notes: |
Abstract An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction.
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| Type of Medium: |
Electronic Resource
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| URL: |