Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942
| ISSN: |
1476-5535
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| Keywords: |
metallothionein ; cyanobacteria ; metals ; bioremediation
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Process Engineering, Biotechnology, Nutrition Technology
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| Notes: |
Abstract A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942.
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| Type of Medium: |
Electronic Resource
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| URL: |