Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942
| ISSN: |
1476-5535
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| Keywords: |
metallothionein ; cyanobacteria ; metals ; bioremediation
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Process Engineering, Biotechnology, Nutrition Technology
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| Notes: |
Abstract A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798296336786784256 |
|---|---|
| autor | Erbe, J L Taylor, K B Hall, L M |
| autorsonst | Erbe, J L Taylor, K B Hall, L M |
| book_url | http://dx.doi.org/10.1007/BF01570147 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM206448430 |
| issn | 1476-5535 |
| journal_name | Journal of industrial microbiology and biotechnology |
| materialart | 1 |
| notes | Abstract A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942. |
| package_name | Springer |
| publikationsjahr_anzeige | 1996 |
| publikationsjahr_facette | 1996 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1996 |
| publisher | Springer |
| reference | 17 (1996), S. 41-46 |
| schlagwort | metallothionein cyanobacteria metals bioremediation |
| search_space | articles |
| shingle_author_1 | Erbe, J L Taylor, K B Hall, L M |
| shingle_author_2 | Erbe, J L Taylor, K B Hall, L M |
| shingle_author_3 | Erbe, J L Taylor, K B Hall, L M |
| shingle_author_4 | Erbe, J L Taylor, K B Hall, L M |
| shingle_catch_all_1 | Erbe, J L Taylor, K B Hall, L M Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 metallothionein cyanobacteria metals bioremediation metallothionein cyanobacteria metals bioremediation Abstract A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942. 1476-5535 14765535 Springer |
| shingle_catch_all_2 | Erbe, J L Taylor, K B Hall, L M Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 metallothionein cyanobacteria metals bioremediation metallothionein cyanobacteria metals bioremediation Abstract A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942. 1476-5535 14765535 Springer |
| shingle_catch_all_3 | Erbe, J L Taylor, K B Hall, L M Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 metallothionein cyanobacteria metals bioremediation metallothionein cyanobacteria metals bioremediation Abstract A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942. 1476-5535 14765535 Springer |
| shingle_catch_all_4 | Erbe, J L Taylor, K B Hall, L M Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 metallothionein cyanobacteria metals bioremediation metallothionein cyanobacteria metals bioremediation Abstract A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942. 1476-5535 14765535 Springer |
| shingle_title_1 | Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 |
| shingle_title_2 | Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 |
| shingle_title_3 | Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 |
| shingle_title_4 | Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:50:29.468Z |
| titel | Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 |
| titel_suche | Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942 |
| topic | W ZM |
| uid | nat_lic_papers_NLM206448430 |