In vitro synthesized immunoglobulin A from nu/+ and reconstituted nu/nu mice against a dominant surface antigen ofGiardia lamblia
| ISSN: |
1432-1955
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Medicine
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| Notes: |
Abstract Nu/+ mice (ZU.ICR-strain) experimentally infected withGiardia lamblia (clone GS/M-83-H7) cleared the infection by day 45 postinfection (p.i.). Athymic nu/nu mice were reconstituted with immune Peyer's patch lymphocytes obtained from self-healed nu/+ littermates and thus acquired the potential to decrease their intestinal parasite mass. Intestinal B-cells from self-healed nu/+ mice as well as from immune-reconstituted athymic nude mice synthesized in vitro parasite-specific immunoglobulin A (IgA). This IgA was subsequently analyzed by immunoblotting, showing a predominant reaction with the major surface antigen (a 72000-Da polypeptide) characterizing theGiardia clone in question. The hypothesis on the causative role of intestinal IgA and immune lymphocytes in the control ofG. lamblia infection thus deserves further attention.
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| Type of Medium: |
Electronic Resource
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| URL: |