Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies
| ISSN: |
1432-1335
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| Keywords: |
Magnetic microspheres ; Cell separation
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Summary The modification and efficiency of a technique for cell separation is described. This technique combines the specificity of serologic reactions with the strength and physical selectivity of a magnetic field. A monoclonal HLA-BW6 antibody was coupled to 400 nm diameter Fe3O4 containing albumin microspheres via surface-incorporated St. aureus Protein-A. The mixture of HLA-BW6 and-BW4 human peripheral blood lymphocytes was incubated with these immunomicropheres and applied to a glass column located in a magnetic field. Only HLA-BW4 lymphocytes passed through the column and were collected. The recovered cells were 97% viable.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295651243524097 |
|---|---|
| autor | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. |
| autorsonst | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. |
| book_url | http://dx.doi.org/10.1007/BF00405076 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM205259499 |
| issn | 1432-1335 |
| journal_name | Journal of cancer research and clinical oncology |
| materialart | 1 |
| notes | Summary The modification and efficiency of a technique for cell separation is described. This technique combines the specificity of serologic reactions with the strength and physical selectivity of a magnetic field. A monoclonal HLA-BW6 antibody was coupled to 400 nm diameter Fe3O4 containing albumin microspheres via surface-incorporated St. aureus Protein-A. The mixture of HLA-BW6 and-BW4 human peripheral blood lymphocytes was incubated with these immunomicropheres and applied to a glass column located in a magnetic field. Only HLA-BW4 lymphocytes passed through the column and were collected. The recovered cells were 97% viable. |
| package_name | Springer |
| publikationsjahr_anzeige | 1981 |
| publikationsjahr_facette | 1981 |
| publikationsjahr_intervall | 8019:1980-1984 |
| publikationsjahr_sort | 1981 |
| publisher | Springer |
| reference | 101 (1981), S. 165-170 |
| schlagwort | Magnetic microspheres Cell separation |
| search_space | articles |
| shingle_author_1 | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. |
| shingle_author_2 | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. |
| shingle_author_3 | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. |
| shingle_author_4 | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. |
| shingle_catch_all_1 | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies Magnetic microspheres Cell separation Magnetic microspheres Cell separation Summary The modification and efficiency of a technique for cell separation is described. This technique combines the specificity of serologic reactions with the strength and physical selectivity of a magnetic field. A monoclonal HLA-BW6 antibody was coupled to 400 nm diameter Fe3O4 containing albumin microspheres via surface-incorporated St. aureus Protein-A. The mixture of HLA-BW6 and-BW4 human peripheral blood lymphocytes was incubated with these immunomicropheres and applied to a glass column located in a magnetic field. Only HLA-BW4 lymphocytes passed through the column and were collected. The recovered cells were 97% viable. 1432-1335 14321335 Springer |
| shingle_catch_all_2 | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies Magnetic microspheres Cell separation Magnetic microspheres Cell separation Summary The modification and efficiency of a technique for cell separation is described. This technique combines the specificity of serologic reactions with the strength and physical selectivity of a magnetic field. A monoclonal HLA-BW6 antibody was coupled to 400 nm diameter Fe3O4 containing albumin microspheres via surface-incorporated St. aureus Protein-A. The mixture of HLA-BW6 and-BW4 human peripheral blood lymphocytes was incubated with these immunomicropheres and applied to a glass column located in a magnetic field. Only HLA-BW4 lymphocytes passed through the column and were collected. The recovered cells were 97% viable. 1432-1335 14321335 Springer |
| shingle_catch_all_3 | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies Magnetic microspheres Cell separation Magnetic microspheres Cell separation Summary The modification and efficiency of a technique for cell separation is described. This technique combines the specificity of serologic reactions with the strength and physical selectivity of a magnetic field. A monoclonal HLA-BW6 antibody was coupled to 400 nm diameter Fe3O4 containing albumin microspheres via surface-incorporated St. aureus Protein-A. The mixture of HLA-BW6 and-BW4 human peripheral blood lymphocytes was incubated with these immunomicropheres and applied to a glass column located in a magnetic field. Only HLA-BW4 lymphocytes passed through the column and were collected. The recovered cells were 97% viable. 1432-1335 14321335 Springer |
| shingle_catch_all_4 | Kandzia, J. Anderson, M. J. D. Müller-Ruchholtz, W. Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies Magnetic microspheres Cell separation Magnetic microspheres Cell separation Summary The modification and efficiency of a technique for cell separation is described. This technique combines the specificity of serologic reactions with the strength and physical selectivity of a magnetic field. A monoclonal HLA-BW6 antibody was coupled to 400 nm diameter Fe3O4 containing albumin microspheres via surface-incorporated St. aureus Protein-A. The mixture of HLA-BW6 and-BW4 human peripheral blood lymphocytes was incubated with these immunomicropheres and applied to a glass column located in a magnetic field. Only HLA-BW4 lymphocytes passed through the column and were collected. The recovered cells were 97% viable. 1432-1335 14321335 Springer |
| shingle_title_1 | Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies |
| shingle_title_2 | Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies |
| shingle_title_3 | Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies |
| shingle_title_4 | Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:39:35.518Z |
| titel | Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies |
| titel_suche | Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM205259499 |