Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies
| ISSN: |
1432-1335
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| Keywords: |
Magnetic microspheres ; Cell separation
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Summary The modification and efficiency of a technique for cell separation is described. This technique combines the specificity of serologic reactions with the strength and physical selectivity of a magnetic field. A monoclonal HLA-BW6 antibody was coupled to 400 nm diameter Fe3O4 containing albumin microspheres via surface-incorporated St. aureus Protein-A. The mixture of HLA-BW6 and-BW4 human peripheral blood lymphocytes was incubated with these immunomicropheres and applied to a glass column located in a magnetic field. Only HLA-BW4 lymphocytes passed through the column and were collected. The recovered cells were 97% viable.
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| Type of Medium: |
Electronic Resource
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| URL: |