Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111
| ISSN: |
1432-0614
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Process Engineering, Biotechnology, Nutrition Technology
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| Notes: |
Summary Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295425920270336 |
|---|---|
| autor | Greiner, M. Winkelmann, G. |
| autorsonst | Greiner, M. Winkelmann, G. |
| book_url | http://dx.doi.org/10.1007/BF00167899 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM202729427 |
| issn | 1432-0614 |
| journal_name | Applied microbiology and biotechnology |
| materialart | 1 |
| notes | Summary Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A. |
| package_name | Springer |
| publikationsjahr_anzeige | 1991 |
| publikationsjahr_facette | 1991 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1991 |
| publisher | Springer |
| reference | 34 (1991), S. 565-569 |
| search_space | articles |
| shingle_author_1 | Greiner, M. Winkelmann, G. |
| shingle_author_2 | Greiner, M. Winkelmann, G. |
| shingle_author_3 | Greiner, M. Winkelmann, G. |
| shingle_author_4 | Greiner, M. Winkelmann, G. |
| shingle_catch_all_1 | Greiner, M. Winkelmann, G. Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 Summary Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A. 1432-0614 14320614 Springer |
| shingle_catch_all_2 | Greiner, M. Winkelmann, G. Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 Summary Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A. 1432-0614 14320614 Springer |
| shingle_catch_all_3 | Greiner, M. Winkelmann, G. Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 Summary Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A. 1432-0614 14320614 Springer |
| shingle_catch_all_4 | Greiner, M. Winkelmann, G. Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 Summary Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A. 1432-0614 14320614 Springer |
| shingle_title_1 | Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 |
| shingle_title_2 | Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 |
| shingle_title_3 | Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 |
| shingle_title_4 | Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:35:58.773Z |
| titel | Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 |
| titel_suche | Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111 |
| topic | W ZM |
| uid | nat_lic_papers_NLM202729427 |