Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111
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1432-0614
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Process Engineering, Biotechnology, Nutrition Technology
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| Notes: |
Summary Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A.
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| Type of Medium: |
Electronic Resource
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| URL: |