Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7
Steinbach, F. ; Alexander, J. ; Tanabe, K. ; Troy, R. ; Edinger, M. G. ; Tubbs, R. R. ; McMahon, J. T. ; Novick, A. C. ; Klein, E. A.
Springer
Published 1995
Springer
Published 1995
| ISSN: |
1434-0879
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|---|---|
| Keywords: |
Kidney neoplasms ; Tumor cells ; Cultures ; Cell adhesion molecules
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Abstract In order to investigate the importance of cell adhesion molecules (CAMs) in renal cell carcinoma (RCC), a cell line, designated as CCF-RC7, was established from a human RCC of the clear cell type. CCF-RC7 was passaged over 50 times in vitro for 31/2 years. The cell line has an epithelial morphology and a doubling time of 30 h, forming colonies in soft agar with an average efficiency of 10.4% and producing clear cell tumors in athymic nude mice. CCF-RC7 cells have an aneuploid-hypotetraploid karyotype with a modal chromosome number of 82 and rearrangements in chromosomes 9, 12 and 14. Immunohistochemical and flow immunocytometric analyses revealed high expression of ICAM-1 (CD54), and Hermes antigen (CD44), which was significantly upregulated by cytokine and PMA treatment. VLA-4 was expressed on approximately 20% of tumor cells and could not be altered by cytokine or PMA stimulation. High expression of sialyl Lewis X was also demonstrated by immunohistological examination. This newly characterized cell line will serve as a useful model for the study of CAMs during hematogenous metastasis and host defense mechanisms in human RCC.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798296059818016768 |
|---|---|
| autor | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. |
| autorsonst | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. |
| book_url | http://dx.doi.org/10.1007/BF00389570 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM202604705 |
| issn | 1434-0879 |
| journal_name | Urological research |
| materialart | 1 |
| notes | Abstract In order to investigate the importance of cell adhesion molecules (CAMs) in renal cell carcinoma (RCC), a cell line, designated as CCF-RC7, was established from a human RCC of the clear cell type. CCF-RC7 was passaged over 50 times in vitro for 31/2 years. The cell line has an epithelial morphology and a doubling time of 30 h, forming colonies in soft agar with an average efficiency of 10.4% and producing clear cell tumors in athymic nude mice. CCF-RC7 cells have an aneuploid-hypotetraploid karyotype with a modal chromosome number of 82 and rearrangements in chromosomes 9, 12 and 14. Immunohistochemical and flow immunocytometric analyses revealed high expression of ICAM-1 (CD54), and Hermes antigen (CD44), which was significantly upregulated by cytokine and PMA treatment. VLA-4 was expressed on approximately 20% of tumor cells and could not be altered by cytokine or PMA stimulation. High expression of sialyl Lewis X was also demonstrated by immunohistological examination. This newly characterized cell line will serve as a useful model for the study of CAMs during hematogenous metastasis and host defense mechanisms in human RCC. |
| package_name | Springer |
| publikationsjahr_anzeige | 1995 |
| publikationsjahr_facette | 1995 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1995 |
| publisher | Springer |
| reference | 23 (1995), S. 175-183 |
| schlagwort | Kidney neoplasms Tumor cells Cultures Cell adhesion molecules |
| search_space | articles |
| shingle_author_1 | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. |
| shingle_author_2 | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. |
| shingle_author_3 | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. |
| shingle_author_4 | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. |
| shingle_catch_all_1 | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 Kidney neoplasms Tumor cells Cultures Cell adhesion molecules Kidney neoplasms Tumor cells Cultures Cell adhesion molecules Abstract In order to investigate the importance of cell adhesion molecules (CAMs) in renal cell carcinoma (RCC), a cell line, designated as CCF-RC7, was established from a human RCC of the clear cell type. CCF-RC7 was passaged over 50 times in vitro for 31/2 years. The cell line has an epithelial morphology and a doubling time of 30 h, forming colonies in soft agar with an average efficiency of 10.4% and producing clear cell tumors in athymic nude mice. CCF-RC7 cells have an aneuploid-hypotetraploid karyotype with a modal chromosome number of 82 and rearrangements in chromosomes 9, 12 and 14. Immunohistochemical and flow immunocytometric analyses revealed high expression of ICAM-1 (CD54), and Hermes antigen (CD44), which was significantly upregulated by cytokine and PMA treatment. VLA-4 was expressed on approximately 20% of tumor cells and could not be altered by cytokine or PMA stimulation. High expression of sialyl Lewis X was also demonstrated by immunohistological examination. This newly characterized cell line will serve as a useful model for the study of CAMs during hematogenous metastasis and host defense mechanisms in human RCC. 1434-0879 14340879 Springer |
| shingle_catch_all_2 | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 Kidney neoplasms Tumor cells Cultures Cell adhesion molecules Kidney neoplasms Tumor cells Cultures Cell adhesion molecules Abstract In order to investigate the importance of cell adhesion molecules (CAMs) in renal cell carcinoma (RCC), a cell line, designated as CCF-RC7, was established from a human RCC of the clear cell type. CCF-RC7 was passaged over 50 times in vitro for 31/2 years. The cell line has an epithelial morphology and a doubling time of 30 h, forming colonies in soft agar with an average efficiency of 10.4% and producing clear cell tumors in athymic nude mice. CCF-RC7 cells have an aneuploid-hypotetraploid karyotype with a modal chromosome number of 82 and rearrangements in chromosomes 9, 12 and 14. Immunohistochemical and flow immunocytometric analyses revealed high expression of ICAM-1 (CD54), and Hermes antigen (CD44), which was significantly upregulated by cytokine and PMA treatment. VLA-4 was expressed on approximately 20% of tumor cells and could not be altered by cytokine or PMA stimulation. High expression of sialyl Lewis X was also demonstrated by immunohistological examination. This newly characterized cell line will serve as a useful model for the study of CAMs during hematogenous metastasis and host defense mechanisms in human RCC. 1434-0879 14340879 Springer |
| shingle_catch_all_3 | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 Kidney neoplasms Tumor cells Cultures Cell adhesion molecules Kidney neoplasms Tumor cells Cultures Cell adhesion molecules Abstract In order to investigate the importance of cell adhesion molecules (CAMs) in renal cell carcinoma (RCC), a cell line, designated as CCF-RC7, was established from a human RCC of the clear cell type. CCF-RC7 was passaged over 50 times in vitro for 31/2 years. The cell line has an epithelial morphology and a doubling time of 30 h, forming colonies in soft agar with an average efficiency of 10.4% and producing clear cell tumors in athymic nude mice. CCF-RC7 cells have an aneuploid-hypotetraploid karyotype with a modal chromosome number of 82 and rearrangements in chromosomes 9, 12 and 14. Immunohistochemical and flow immunocytometric analyses revealed high expression of ICAM-1 (CD54), and Hermes antigen (CD44), which was significantly upregulated by cytokine and PMA treatment. VLA-4 was expressed on approximately 20% of tumor cells and could not be altered by cytokine or PMA stimulation. High expression of sialyl Lewis X was also demonstrated by immunohistological examination. This newly characterized cell line will serve as a useful model for the study of CAMs during hematogenous metastasis and host defense mechanisms in human RCC. 1434-0879 14340879 Springer |
| shingle_catch_all_4 | Steinbach, F. Alexander, J. Tanabe, K. Troy, R. Edinger, M. G. Tubbs, R. R. McMahon, J. T. Novick, A. C. Klein, E. A. Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 Kidney neoplasms Tumor cells Cultures Cell adhesion molecules Kidney neoplasms Tumor cells Cultures Cell adhesion molecules Abstract In order to investigate the importance of cell adhesion molecules (CAMs) in renal cell carcinoma (RCC), a cell line, designated as CCF-RC7, was established from a human RCC of the clear cell type. CCF-RC7 was passaged over 50 times in vitro for 31/2 years. The cell line has an epithelial morphology and a doubling time of 30 h, forming colonies in soft agar with an average efficiency of 10.4% and producing clear cell tumors in athymic nude mice. CCF-RC7 cells have an aneuploid-hypotetraploid karyotype with a modal chromosome number of 82 and rearrangements in chromosomes 9, 12 and 14. Immunohistochemical and flow immunocytometric analyses revealed high expression of ICAM-1 (CD54), and Hermes antigen (CD44), which was significantly upregulated by cytokine and PMA treatment. VLA-4 was expressed on approximately 20% of tumor cells and could not be altered by cytokine or PMA stimulation. High expression of sialyl Lewis X was also demonstrated by immunohistological examination. This newly characterized cell line will serve as a useful model for the study of CAMs during hematogenous metastasis and host defense mechanisms in human RCC. 1434-0879 14340879 Springer |
| shingle_title_1 | Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 |
| shingle_title_2 | Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 |
| shingle_title_3 | Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 |
| shingle_title_4 | Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:46:05.254Z |
| titel | Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 |
| titel_suche | Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7 |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM202604705 |