Expression of cell adhesion molecules in an established and characterized new human renal cell cancer line, CCF-RC7
Steinbach, F. ; Alexander, J. ; Tanabe, K. ; Troy, R. ; Edinger, M. G. ; Tubbs, R. R. ; McMahon, J. T. ; Novick, A. C. ; Klein, E. A.
Springer
Published 1995
Springer
Published 1995
| ISSN: |
1434-0879
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|---|---|
| Keywords: |
Kidney neoplasms ; Tumor cells ; Cultures ; Cell adhesion molecules
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Abstract In order to investigate the importance of cell adhesion molecules (CAMs) in renal cell carcinoma (RCC), a cell line, designated as CCF-RC7, was established from a human RCC of the clear cell type. CCF-RC7 was passaged over 50 times in vitro for 31/2 years. The cell line has an epithelial morphology and a doubling time of 30 h, forming colonies in soft agar with an average efficiency of 10.4% and producing clear cell tumors in athymic nude mice. CCF-RC7 cells have an aneuploid-hypotetraploid karyotype with a modal chromosome number of 82 and rearrangements in chromosomes 9, 12 and 14. Immunohistochemical and flow immunocytometric analyses revealed high expression of ICAM-1 (CD54), and Hermes antigen (CD44), which was significantly upregulated by cytokine and PMA treatment. VLA-4 was expressed on approximately 20% of tumor cells and could not be altered by cytokine or PMA stimulation. High expression of sialyl Lewis X was also demonstrated by immunohistological examination. This newly characterized cell line will serve as a useful model for the study of CAMs during hematogenous metastasis and host defense mechanisms in human RCC.
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| Type of Medium: |
Electronic Resource
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| URL: |