Estrogen receptors in 699 primary breast cancers: A comparison of immunohistochemical and biochemical methods
Molino, A. ; Micciolo, R. ; Turazza, M. ; Bonetti, F. ; Piubello, Q. ; Corgnati, A. ; Sperotto, L. ; Martignoni, G. ; Bonetti, A. ; Nortilli, R. ; Castelli, P. ; Rodella, S. ; Capelli, P. ; Manfrin, E. ; Pelosi, G. ; Cetto, G. L.
Springer
Published 1995
Springer
Published 1995
| ISSN: |
1573-7217
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|---|---|
| Keywords: |
biochemistry ; breast cancer ; estrogen receptors ; immunohistochemistry
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Summary Background: Over the last few years, estrogen receptor (ER) determination by immunohistochemistry (ER-ICA) has been extensively used, but it still remains to be established whether this method can replace the standard biochemical technique using dextran-coated charcoal (ERDCC). Patients and methods: ER were determined by both the dextran-coated charcoal (DCC) method and immunohistochemistry (ICA) in 699 patients with primary breast cancer; other parameters (age, pathological T-pT- and nodal status -pN-, progesterone receptors by DCC, proliferative index by ICA) were also recorded. The ‘best’ cut-off for ERICA was evaluated by means of Receiver Operating Characteristics (R.O.C.) analysis; logistic regression analysis was used to find adequate ‘weights’ for stain intensity. Results and conclusions: A significant correlation was found between the two methods (p 〈 0.001). R.O.C. analysis revealed that the ‘best’ cut-off for the ERICA score was 45% (sensitivity 0.810, specificity 0.804). Logistic regression analysis showed that an ERICA score which also considers staining intensity does not add any useful information concerning ER content in breast cancers.
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| Type of Medium: |
Electronic Resource
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| URL: |