Development of SCAR markers linked to the Ns locus in potato
| ISSN: |
1439-0523
|
|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
|
| Topics: |
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
|
| Notes: |
A random amplified polymorphic DNA marker OPG17450 linked to the Ns gene that confers resistance of potato to potato virus S (PVS), was used to develop sequence-characterized amplified region (SCAR) markers. After cloning and sequencing of OPG17450 new polymerase chain reaction (PCR) primers were designed to generate dominant (SCG17321) and codominant (SCG17448) markers. For SCG17448, polymorphism between susceptible and resistant genotypes was recovered after digestion of the marker with the restriction enzyme Muni. In addition to the band corresponding to ‘susceptible’ allele that does not contain the Muni cleavage site, two bands of approximately 251 bp and 197 bp were observed in the resistant genotypes. The usefulness of these SCAR markers was verified in diploid potatoes possessing the Ns locus from clone G-LKS 678147/60, and in tetraploid potatoes derived from G-LKS 678147/60 and from clone MPI 65118/3.
|
| Type of Medium: |
Electronic Resource
|
| URL: |