The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice
Chen, Y.-Y. ; Suen, J.-L. ; Wu, W.-M. ; Chiang, B.-L.
Oxford, UK : Blackwell Science Ltd
Published 2001
Oxford, UK : Blackwell Science Ltd
Published 2001
| ISSN: |
1365-3083
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|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Programmed cell death (apoptosis) is an essential process in the development of various tissues and its involvement has been proposed for the elimination of self-reactive immature T and B lymphocytes when self antigens are first encountered. In order to further investigate the role of apoptosis in the pathogenesis of autoimmune disease, the apoptosis of lipopolysaccharide (LPS)-activated B cells, peritoneal cells from NZB x NZW F1 (NZB/W F1) mice and nonautoimmune BALB/c mice were assayed using an in vitro culture system. Splenic B cells were isolated and then stimulated with LPS before further activated with crosslinking antiµ antibody. In addition, the apoptosis of peritoneal cells induced by crosslinking antiµ antibody was also analyzed. The data revealed that the specific apoptosis of both activated B cells and peritoneal cells induced by crosslinking antiµ antibody was very similar comparing NZB/W F1 and nonautoimmune BALB/c mice. This activation-induced B-cells apoptosis could be rescued, however, with the addition of cytokines such as interleukin (IL)-5 or IL-10, to the culture. The results suggest that there is no endogenous defect in the apoptosis of activated B cells for autoimmune NZB/W F1 comparing nonautoimmune BALB/c mice. Notably, however, abnormally high levels of the type 2 T helper (Th2)-related cytokines such as IL-5 or IL-10 may play an important role in the abnormal expansion of activated B cells in autoimmune NZB/W F1 mice.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798290232263573506 |
|---|---|
| autor | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. |
| autorsonst | Chiang, B.-L. |
| book_url | http://dx.doi.org/10.1046/j.1365-3083.2001.00922.x |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLZ243703082 |
| insertion_date | 2012-04-27 |
| issn | 1365-3083 |
| journal_name | Scandinavian journal of immunology |
| materialart | 1 |
| notes | Programmed cell death (apoptosis) is an essential process in the development of various tissues and its involvement has been proposed for the elimination of self-reactive immature T and B lymphocytes when self antigens are first encountered. In order to further investigate the role of apoptosis in the pathogenesis of autoimmune disease, the apoptosis of lipopolysaccharide (LPS)-activated B cells, peritoneal cells from NZB x NZW F1 (NZB/W F1) mice and nonautoimmune BALB/c mice were assayed using an in vitro culture system. Splenic B cells were isolated and then stimulated with LPS before further activated with crosslinking antiµ antibody. In addition, the apoptosis of peritoneal cells induced by crosslinking antiµ antibody was also analyzed. The data revealed that the specific apoptosis of both activated B cells and peritoneal cells induced by crosslinking antiµ antibody was very similar comparing NZB/W F1 and nonautoimmune BALB/c mice. This activation-induced B-cells apoptosis could be rescued, however, with the addition of cytokines such as interleukin (IL)-5 or IL-10, to the culture. The results suggest that there is no endogenous defect in the apoptosis of activated B cells for autoimmune NZB/W F1 comparing nonautoimmune BALB/c mice. Notably, however, abnormally high levels of the type 2 T helper (Th2)-related cytokines such as IL-5 or IL-10 may play an important role in the abnormal expansion of activated B cells in autoimmune NZB/W F1 mice. |
| package_name | Blackwell Publishing |
| publikationsjahr_anzeige | 2001 |
| publikationsjahr_facette | 2001 |
| publikationsjahr_intervall | 7999:2000-2004 |
| publikationsjahr_sort | 2001 |
| publikationsort | Oxford, UK |
| publisher | Blackwell Science Ltd |
| reference | 53 (2001), S. 0 |
| search_space | articles |
| shingle_author_1 | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. |
| shingle_author_2 | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. |
| shingle_author_3 | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. |
| shingle_author_4 | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. |
| shingle_catch_all_1 | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice Blackwell Science Ltd Programmed cell death (apoptosis) is an essential process in the development of various tissues and its involvement has been proposed for the elimination of self-reactive immature T and B lymphocytes when self antigens are first encountered. In order to further investigate the role of apoptosis in the pathogenesis of autoimmune disease, the apoptosis of lipopolysaccharide (LPS)-activated B cells, peritoneal cells from NZB x NZW F1 (NZB/W F1) mice and nonautoimmune BALB/c mice were assayed using an in vitro culture system. Splenic B cells were isolated and then stimulated with LPS before further activated with crosslinking antiµ antibody. In addition, the apoptosis of peritoneal cells induced by crosslinking antiµ antibody was also analyzed. The data revealed that the specific apoptosis of both activated B cells and peritoneal cells induced by crosslinking antiµ antibody was very similar comparing NZB/W F1 and nonautoimmune BALB/c mice. This activation-induced B-cells apoptosis could be rescued, however, with the addition of cytokines such as interleukin (IL)-5 or IL-10, to the culture. The results suggest that there is no endogenous defect in the apoptosis of activated B cells for autoimmune NZB/W F1 comparing nonautoimmune BALB/c mice. Notably, however, abnormally high levels of the type 2 T helper (Th2)-related cytokines such as IL-5 or IL-10 may play an important role in the abnormal expansion of activated B cells in autoimmune NZB/W F1 mice. 1365-3083 13653083 |
| shingle_catch_all_2 | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice Blackwell Science Ltd Programmed cell death (apoptosis) is an essential process in the development of various tissues and its involvement has been proposed for the elimination of self-reactive immature T and B lymphocytes when self antigens are first encountered. In order to further investigate the role of apoptosis in the pathogenesis of autoimmune disease, the apoptosis of lipopolysaccharide (LPS)-activated B cells, peritoneal cells from NZB x NZW F1 (NZB/W F1) mice and nonautoimmune BALB/c mice were assayed using an in vitro culture system. Splenic B cells were isolated and then stimulated with LPS before further activated with crosslinking antiµ antibody. In addition, the apoptosis of peritoneal cells induced by crosslinking antiµ antibody was also analyzed. The data revealed that the specific apoptosis of both activated B cells and peritoneal cells induced by crosslinking antiµ antibody was very similar comparing NZB/W F1 and nonautoimmune BALB/c mice. This activation-induced B-cells apoptosis could be rescued, however, with the addition of cytokines such as interleukin (IL)-5 or IL-10, to the culture. The results suggest that there is no endogenous defect in the apoptosis of activated B cells for autoimmune NZB/W F1 comparing nonautoimmune BALB/c mice. Notably, however, abnormally high levels of the type 2 T helper (Th2)-related cytokines such as IL-5 or IL-10 may play an important role in the abnormal expansion of activated B cells in autoimmune NZB/W F1 mice. 1365-3083 13653083 |
| shingle_catch_all_3 | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice Blackwell Science Ltd Programmed cell death (apoptosis) is an essential process in the development of various tissues and its involvement has been proposed for the elimination of self-reactive immature T and B lymphocytes when self antigens are first encountered. In order to further investigate the role of apoptosis in the pathogenesis of autoimmune disease, the apoptosis of lipopolysaccharide (LPS)-activated B cells, peritoneal cells from NZB x NZW F1 (NZB/W F1) mice and nonautoimmune BALB/c mice were assayed using an in vitro culture system. Splenic B cells were isolated and then stimulated with LPS before further activated with crosslinking antiµ antibody. In addition, the apoptosis of peritoneal cells induced by crosslinking antiµ antibody was also analyzed. The data revealed that the specific apoptosis of both activated B cells and peritoneal cells induced by crosslinking antiµ antibody was very similar comparing NZB/W F1 and nonautoimmune BALB/c mice. This activation-induced B-cells apoptosis could be rescued, however, with the addition of cytokines such as interleukin (IL)-5 or IL-10, to the culture. The results suggest that there is no endogenous defect in the apoptosis of activated B cells for autoimmune NZB/W F1 comparing nonautoimmune BALB/c mice. Notably, however, abnormally high levels of the type 2 T helper (Th2)-related cytokines such as IL-5 or IL-10 may play an important role in the abnormal expansion of activated B cells in autoimmune NZB/W F1 mice. 1365-3083 13653083 |
| shingle_catch_all_4 | Chen, Y.-Y. Suen, J.-L. Wu, W.-M. Chiang, B.-L. The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice Blackwell Science Ltd Programmed cell death (apoptosis) is an essential process in the development of various tissues and its involvement has been proposed for the elimination of self-reactive immature T and B lymphocytes when self antigens are first encountered. In order to further investigate the role of apoptosis in the pathogenesis of autoimmune disease, the apoptosis of lipopolysaccharide (LPS)-activated B cells, peritoneal cells from NZB x NZW F1 (NZB/W F1) mice and nonautoimmune BALB/c mice were assayed using an in vitro culture system. Splenic B cells were isolated and then stimulated with LPS before further activated with crosslinking antiµ antibody. In addition, the apoptosis of peritoneal cells induced by crosslinking antiµ antibody was also analyzed. The data revealed that the specific apoptosis of both activated B cells and peritoneal cells induced by crosslinking antiµ antibody was very similar comparing NZB/W F1 and nonautoimmune BALB/c mice. This activation-induced B-cells apoptosis could be rescued, however, with the addition of cytokines such as interleukin (IL)-5 or IL-10, to the culture. The results suggest that there is no endogenous defect in the apoptosis of activated B cells for autoimmune NZB/W F1 comparing nonautoimmune BALB/c mice. Notably, however, abnormally high levels of the type 2 T helper (Th2)-related cytokines such as IL-5 or IL-10 may play an important role in the abnormal expansion of activated B cells in autoimmune NZB/W F1 mice. 1365-3083 13653083 |
| shingle_title_1 | The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice |
| shingle_title_2 | The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice |
| shingle_title_3 | The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice |
| shingle_title_4 | The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice |
| sigel_instance_filter | dkfz geomar wilbert ipn albert |
| source_archive | Blackwell Publishing Journal Backfiles 1879-2005 |
| timestamp | 2024-05-06T08:13:27.252Z |
| titel | The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice |
| titel_suche | The Effect of Cytokines on the Activation-Induced Apoptosis of B Cells in Autoimmune NZB X NZW F1 Mice |
| topic | WW-YZ |
| uid | nat_lic_papers_NLZ243703082 |