Ontogeny of the Murine Ig Joining Chain Gene and Protein
Kimura, M. ; Takahashi, T. ; Iwata, A. ; Matsumoto, N. ; Ogura, Y. ; Akagi, T. ; Akima, S. ; Kobayashi, K. ; Moro, I.
Oxford, UK : Blackwell Science Ltd
Published 2001
Oxford, UK : Blackwell Science Ltd
Published 2001
| ISSN: |
1365-3083
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
We used Northern blot analysis in order to investigate the ontogeny of the murine joining (J)-chain gene. No J-chain expression was detected in embryonic tissues, including liver, spleen and intestine, but an expression of µ heavy chain was detected in foetal liver at day 17. J-chain expression was detected in the spleen at day 9 and in the intestine at day 15 after birth. Western blot analysis was carried out in order to compare the protein levels of J and µ heavy chains in serum from day 8 to day 24 after birth, using antihuman J chain and antimouse µ chain antibodies. Although µ chain protein could be detected in serum from day 8, J-chain protein was detectable only at day 24. These results suggest that the expression of J chain is a later event than the µ chain in the mouse, which thus differs in embryogenesis from humans.
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| Type of Medium: |
Electronic Resource
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| URL: |