Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing
Diegel, M. L. ; Chen, F. ; Laus, R. ; Graddis, T. J. ; Vidovic, D.
Oxford, UK : Blackwell Science Ltd
Published 2003
Oxford, UK : Blackwell Science Ltd
Published 2003
| ISSN: |
1365-3083
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|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Proteins in their native form are incapable of stimulating antigen (Ag)-specific T cells, which can only recognize major histocompatibility complex (MHC)-bound peptides that have been generated by intracellular processing within antigen-presenting cells (APCs). Here, we show that APCs can trigger MHC class I-restricted T-cell responses after presenting proteins without conventional intracellular processing, provided the immunostimulatory MHC class I-binding peptide sequence is incorporated at the carboxy-terminal position. Such MHC-bound proteins do not stimulate T cells directly, because the contact between MHC/peptide complex and its cognate ligand is sterically hindered by the amino-terminal bulk of the protein. Removal of the latter via an extracellular Ag proteolysis by the T-cell- and/or APC-derived enzymes is required for effective T-cell stimulation. Our data challenge the established concept that only small peptides can bind to the MHC class I molecules.
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| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798290232184930304 |
|---|---|
| autor | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. |
| autorsonst | Graddis, T. J. Vidovic, D. |
| book_url | http://dx.doi.org/10.1046/j.1365-3083.2003.01252.x |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLZ243699417 |
| insertion_date | 2012-04-27 |
| issn | 1365-3083 |
| journal_name | Scandinavian journal of immunology |
| materialart | 1 |
| notes | Proteins in their native form are incapable of stimulating antigen (Ag)-specific T cells, which can only recognize major histocompatibility complex (MHC)-bound peptides that have been generated by intracellular processing within antigen-presenting cells (APCs). Here, we show that APCs can trigger MHC class I-restricted T-cell responses after presenting proteins without conventional intracellular processing, provided the immunostimulatory MHC class I-binding peptide sequence is incorporated at the carboxy-terminal position. Such MHC-bound proteins do not stimulate T cells directly, because the contact between MHC/peptide complex and its cognate ligand is sterically hindered by the amino-terminal bulk of the protein. Removal of the latter via an extracellular Ag proteolysis by the T-cell- and/or APC-derived enzymes is required for effective T-cell stimulation. Our data challenge the established concept that only small peptides can bind to the MHC class I molecules. |
| package_name | Blackwell Publishing |
| publikationsjahr_anzeige | 2003 |
| publikationsjahr_facette | 2003 |
| publikationsjahr_intervall | 7999:2000-2004 |
| publikationsjahr_sort | 2003 |
| publikationsort | Oxford, UK |
| publisher | Blackwell Science Ltd |
| reference | 58 (2003), S. 0 |
| search_space | articles |
| shingle_author_1 | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. |
| shingle_author_2 | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. |
| shingle_author_3 | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. |
| shingle_author_4 | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. |
| shingle_catch_all_1 | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing Blackwell Science Ltd Proteins in their native form are incapable of stimulating antigen (Ag)-specific T cells, which can only recognize major histocompatibility complex (MHC)-bound peptides that have been generated by intracellular processing within antigen-presenting cells (APCs). Here, we show that APCs can trigger MHC class I-restricted T-cell responses after presenting proteins without conventional intracellular processing, provided the immunostimulatory MHC class I-binding peptide sequence is incorporated at the carboxy-terminal position. Such MHC-bound proteins do not stimulate T cells directly, because the contact between MHC/peptide complex and its cognate ligand is sterically hindered by the amino-terminal bulk of the protein. Removal of the latter via an extracellular Ag proteolysis by the T-cell- and/or APC-derived enzymes is required for effective T-cell stimulation. Our data challenge the established concept that only small peptides can bind to the MHC class I molecules. 1365-3083 13653083 |
| shingle_catch_all_2 | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing Blackwell Science Ltd Proteins in their native form are incapable of stimulating antigen (Ag)-specific T cells, which can only recognize major histocompatibility complex (MHC)-bound peptides that have been generated by intracellular processing within antigen-presenting cells (APCs). Here, we show that APCs can trigger MHC class I-restricted T-cell responses after presenting proteins without conventional intracellular processing, provided the immunostimulatory MHC class I-binding peptide sequence is incorporated at the carboxy-terminal position. Such MHC-bound proteins do not stimulate T cells directly, because the contact between MHC/peptide complex and its cognate ligand is sterically hindered by the amino-terminal bulk of the protein. Removal of the latter via an extracellular Ag proteolysis by the T-cell- and/or APC-derived enzymes is required for effective T-cell stimulation. Our data challenge the established concept that only small peptides can bind to the MHC class I molecules. 1365-3083 13653083 |
| shingle_catch_all_3 | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing Blackwell Science Ltd Proteins in their native form are incapable of stimulating antigen (Ag)-specific T cells, which can only recognize major histocompatibility complex (MHC)-bound peptides that have been generated by intracellular processing within antigen-presenting cells (APCs). Here, we show that APCs can trigger MHC class I-restricted T-cell responses after presenting proteins without conventional intracellular processing, provided the immunostimulatory MHC class I-binding peptide sequence is incorporated at the carboxy-terminal position. Such MHC-bound proteins do not stimulate T cells directly, because the contact between MHC/peptide complex and its cognate ligand is sterically hindered by the amino-terminal bulk of the protein. Removal of the latter via an extracellular Ag proteolysis by the T-cell- and/or APC-derived enzymes is required for effective T-cell stimulation. Our data challenge the established concept that only small peptides can bind to the MHC class I molecules. 1365-3083 13653083 |
| shingle_catch_all_4 | Diegel, M. L. Chen, F. Laus, R. Graddis, T. J. Vidovic, D. Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing Blackwell Science Ltd Proteins in their native form are incapable of stimulating antigen (Ag)-specific T cells, which can only recognize major histocompatibility complex (MHC)-bound peptides that have been generated by intracellular processing within antigen-presenting cells (APCs). Here, we show that APCs can trigger MHC class I-restricted T-cell responses after presenting proteins without conventional intracellular processing, provided the immunostimulatory MHC class I-binding peptide sequence is incorporated at the carboxy-terminal position. Such MHC-bound proteins do not stimulate T cells directly, because the contact between MHC/peptide complex and its cognate ligand is sterically hindered by the amino-terminal bulk of the protein. Removal of the latter via an extracellular Ag proteolysis by the T-cell- and/or APC-derived enzymes is required for effective T-cell stimulation. Our data challenge the established concept that only small peptides can bind to the MHC class I molecules. 1365-3083 13653083 |
| shingle_title_1 | Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing |
| shingle_title_2 | Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing |
| shingle_title_3 | Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing |
| shingle_title_4 | Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing |
| sigel_instance_filter | dkfz geomar wilbert ipn albert |
| source_archive | Blackwell Publishing Journal Backfiles 1879-2005 |
| timestamp | 2024-05-06T08:13:27.202Z |
| titel | Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing |
| titel_suche | Major Histocompatibility Complex Class I-Restricted Presentation of Protein Antigens without Prior Intracellular Processing |
| topic | WW-YZ |
| uid | nat_lic_papers_NLZ243699417 |