T Helper 2 Immunity to Hepatitis B Surface Antigen Primed by Gene-Gun-Mediated DNA Vaccination can be Shifted Towards T helper 1 Immunity by Codelivery of CpG Motif-Containing Oligodeoxynucleotides
Zhou, X. ; Zheng, L. ; Liu, L. ; Xiang, L. ; Yuan, Z.
Oxford, UK : Blackwell Science Ltd
Published 2003
Oxford, UK : Blackwell Science Ltd
Published 2003
| ISSN: |
1365-3083
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Gene-gun-mediated DNA immunization usually induces predominant T helper 2 (Th2) type immune response. As oligodeoxynucleotides (ODN)-containing unmethylated CpG motifs can activate the innate immune system in a Th1-biased way, the potential of codelivery of CpG motifs-containing ODN (CpG-ODN) with plasmid DNA to switch the gene-gun-mediated Th2 immune response was evaluated in this study. Here we show that codelivery of CpG-ODN with plasmid DNA at certain ratio (10/1) can enhance the Th1 humoral and cell-mediated immune responses in gene-gun-mediated DNA immunization in BALB/c mice, including increasing the hepatitis B surface antigen-specific total immunoglobulin G (IgG), IgG2a subclass, cytotoxic T-cell lymphocyte activity as well as interferon-γ (IFN-γ) secretion. Taken together, these results demonstrate that codelivery of CpG-ODN with recombinant plasmid DNA by gene gun can shift the gene-gun-mediated DNA immune response from Th2 towards Th1.
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| Type of Medium: |
Electronic Resource
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| URL: |