061 Lactate, Oxygen, and Wound Healing
Aslam, R. ; Scheuenstuhl, H. ; Hopf, H. ; Beckert, S. ; Hussain, Z. ; Hunt, T.K.
Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
Published 2005
Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
Published 2005
| ISSN: |
1524-475X
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Hypothesis: Both hypoxia and lactate independently induce VEGF synthesis. However, an adequate supply of oxygen is required for new vessels to mature.Materials and Methods: We used in vivo matrigel (growth factor reduced) angiogenesis assay and Swiss Webster mice n = 36 (3 groups of 12 with 6 control and 6 experimental in each). Each mouse received a 1 ml subcutaneous matrigel injection. Matrigel solidifies into a gel implant when injected subcutaneously. Control injections/implants were matrigel with no added substance and experimental implants were matrigel supplemented with 30 mgs of crushed lactate polymer or PL (poly DL-Lactide-co-Glycolide 50:50; av. mol.wt. 50–75,000 kilo Daltons). The first group of mice was maintained in 12–14% oxygen, the second group in 21% oxygen, and the third group in 50–52% oxygen continuously for 11 days, at which time the implants were harvested, fixed, sectioned, and stained with Mason’s Trichrome to identify new vessels and collagen.Results: 1. 13% oxygen control: Half showed new blood vessels with thin or incomplete walls and hemorrhages. Few collagen fibers2. 13% oxygen with PL: same as above3. 21% oxygen control: none showed new blood vessels/collagen fibers4. 21% oxygen with PL: five out of six showed new blood vessels. Almost all were mature, with complete walls, collagen fibers, and no hemorrhages5. 51% oxygen control: None showed new blood vessels/collagen fibers6. 51% oxygen with PL: all six developed new vessels with some hemorrhage seen in two out of six. Collagen fibers presentNote: matrigel contains collagen and stained a pale blue, collagen fibers were seen as dark blue, endothelial cells and RBCs as dark red and red. All blood vessels contained RBCs.Conclusions: Hypoxia and lactate induce VEGF. However, hypoxia limits vascular growth by inhibiting collagen deposition. The largest and most mature new vessels result from a combination of oxygen and lactate.
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| Type of Medium: |
Electronic Resource
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| URL: |