Immediate GTP hydrolysis upon FtsZ polymerization
ISSN: |
1365-2958
|
---|---|
Source: |
Blackwell Publishing Journal Backfiles 1879-2005
|
Topics: |
Biology
Medicine
|
Notes: |
To understand the polymerization dynamics of FtsZ, a bacterial cell division protein similar to tubulin, insight is required into the nature of the nucleotide bound to the polymerized protein. In a previous study, we showed that the FtsZ polymers contain mostly GDP. A recent study challenged this result, suggesting that the polymerized FtsZ is in a GTP-bound state. Here, we show that, when radiolabelled [γ-32P]-GTP is used to polymerize FtsZ, GTP is hydrolysed instantaneously. The FtsZ polymer contains both GDP and the radiolabelled inorganic phosphate.
|
Type of Medium: |
Electronic Resource
|
URL: |