DNA Polymorphism of Percidae population structure in the Kuibyshev water reservoir of Russia

1095-8649

Blackwell Publishing Journal Backfiles 1879-2005

Biology

The pike perches Stizostedion lucioperca and S. volgense, river perch Perca fluviatilis and ruffe Gymnocephalus cernuus are important components of the fish fauna of the Kuibyshev reservoir in Russia. We analysed genetic variation within and among populations of these species. Two microsatellite DNA from walleye S. vitreum were used as primers of this study. Each of the DNA markers resolved 6–10 alleles with locus Svi4 and Svi6 and size range of 102–169 base pairs (bp). PCR was performed with the selected primers, with temperature cycles followed a denaturing profile for 30 s at 94° C. A final 10 min elongation step was resumed at 72° C after 35 cycles. Stizostedion volgense showed the highest bp of 100 to 3000, differing by sex, age and maturity level. The populations of P. fluviatilis have variable DNA polymorphism within the range 90–1000 base pairs. We also detected a new band in the fingerprints. The degree of neutral genetic variation detected indicates that effective population sizes of the study species are large.

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