Properties of Liposomes Prepared with Various Lipids

Hsieh, Y.-F. ; Chen, T.-L. ; Wang, Y.-T. ; Chang, J.-H. ; Chang, H.-M.

Oxford, UK : Blackwell Publishing Ltd
Published 2002
ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Process Engineering, Biotechnology, Nutrition Technology
Notes:
: Use of several lipids as an alternative to cholesterol to prepare stable liposomes was tried. Liposome prepared at 1/0.25 lecithin/stearic acid ratio exhibited better (55%) encapsulation efficiency (EE) of bovine serum albumin (BSA) than that (41%) prepared at 1/0.25 lecithin/cholesterol ratio. Liposomes showed the minimal released percentage of encapsulated BSA at pH 6. Storage at −20 °C caused serious damage to liposomes. Addition of α–tocopherol was effective in stabilizing liposomes. Liposomes, incorporated with α–amylase, prepared at 1/0.25 lecithin/cholesterol or stearic acid ratio were effective in protecting enzyme against acid (pH 2.8) and pepsin (15 mg/mL). Use of stearic acid, instead of cholesterol, would be feasible in preparing stable liposomes.
Type of Medium:
Electronic Resource
URL:
_version_ 1798290358959865857
autor Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
autorsonst Chang, J.-H.
Chang, H.-M.
book_url http://dx.doi.org/10.1111/j.1365-2621.2002.tb08820.x
datenlieferant nat_lic_papers
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insertion_date 2012-04-27
issn 1750-3841
journal_name Journal of food science
materialart 1
notes : Use of several lipids as an alternative to cholesterol to prepare stable liposomes was tried. Liposome prepared at 1/0.25 lecithin/stearic acid ratio exhibited better (55%) encapsulation efficiency (EE) of bovine serum albumin (BSA) than that (41%) prepared at 1/0.25 lecithin/cholesterol ratio. Liposomes showed the minimal released percentage of encapsulated BSA at pH 6. Storage at −20 °C caused serious damage to liposomes. Addition of α–tocopherol was effective in stabilizing liposomes. Liposomes, incorporated with α–amylase, prepared at 1/0.25 lecithin/cholesterol or stearic acid ratio were effective in protecting enzyme against acid (pH 2.8) and pepsin (15 mg/mL). Use of stearic acid, instead of cholesterol, would be feasible in preparing stable liposomes.
package_name Blackwell Publishing
publikationsjahr_anzeige 2002
publikationsjahr_facette 2002
publikationsjahr_intervall 7999:2000-2004
publikationsjahr_sort 2002
publikationsort Oxford, UK
publisher Blackwell Publishing Ltd
reference 67 (2002), S. 0
search_space articles
shingle_author_1 Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
shingle_author_2 Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
shingle_author_3 Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
shingle_author_4 Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
shingle_catch_all_1 Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
Properties of Liposomes Prepared with Various Lipids
Blackwell Publishing Ltd
: Use of several lipids as an alternative to cholesterol to prepare stable liposomes was tried. Liposome prepared at 1/0.25 lecithin/stearic acid ratio exhibited better (55%) encapsulation efficiency (EE) of bovine serum albumin (BSA) than that (41%) prepared at 1/0.25 lecithin/cholesterol ratio. Liposomes showed the minimal released percentage of encapsulated BSA at pH 6. Storage at −20 °C caused serious damage to liposomes. Addition of α–tocopherol was effective in stabilizing liposomes. Liposomes, incorporated with α–amylase, prepared at 1/0.25 lecithin/cholesterol or stearic acid ratio were effective in protecting enzyme against acid (pH 2.8) and pepsin (15 mg/mL). Use of stearic acid, instead of cholesterol, would be feasible in preparing stable liposomes.
1750-3841
17503841
shingle_catch_all_2 Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
Properties of Liposomes Prepared with Various Lipids
Blackwell Publishing Ltd
: Use of several lipids as an alternative to cholesterol to prepare stable liposomes was tried. Liposome prepared at 1/0.25 lecithin/stearic acid ratio exhibited better (55%) encapsulation efficiency (EE) of bovine serum albumin (BSA) than that (41%) prepared at 1/0.25 lecithin/cholesterol ratio. Liposomes showed the minimal released percentage of encapsulated BSA at pH 6. Storage at −20 °C caused serious damage to liposomes. Addition of α–tocopherol was effective in stabilizing liposomes. Liposomes, incorporated with α–amylase, prepared at 1/0.25 lecithin/cholesterol or stearic acid ratio were effective in protecting enzyme against acid (pH 2.8) and pepsin (15 mg/mL). Use of stearic acid, instead of cholesterol, would be feasible in preparing stable liposomes.
1750-3841
17503841
shingle_catch_all_3 Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
Properties of Liposomes Prepared with Various Lipids
Blackwell Publishing Ltd
: Use of several lipids as an alternative to cholesterol to prepare stable liposomes was tried. Liposome prepared at 1/0.25 lecithin/stearic acid ratio exhibited better (55%) encapsulation efficiency (EE) of bovine serum albumin (BSA) than that (41%) prepared at 1/0.25 lecithin/cholesterol ratio. Liposomes showed the minimal released percentage of encapsulated BSA at pH 6. Storage at −20 °C caused serious damage to liposomes. Addition of α–tocopherol was effective in stabilizing liposomes. Liposomes, incorporated with α–amylase, prepared at 1/0.25 lecithin/cholesterol or stearic acid ratio were effective in protecting enzyme against acid (pH 2.8) and pepsin (15 mg/mL). Use of stearic acid, instead of cholesterol, would be feasible in preparing stable liposomes.
1750-3841
17503841
shingle_catch_all_4 Hsieh, Y.-F.
Chen, T.-L.
Wang, Y.-T.
Chang, J.-H.
Chang, H.-M.
Properties of Liposomes Prepared with Various Lipids
Blackwell Publishing Ltd
: Use of several lipids as an alternative to cholesterol to prepare stable liposomes was tried. Liposome prepared at 1/0.25 lecithin/stearic acid ratio exhibited better (55%) encapsulation efficiency (EE) of bovine serum albumin (BSA) than that (41%) prepared at 1/0.25 lecithin/cholesterol ratio. Liposomes showed the minimal released percentage of encapsulated BSA at pH 6. Storage at −20 °C caused serious damage to liposomes. Addition of α–tocopherol was effective in stabilizing liposomes. Liposomes, incorporated with α–amylase, prepared at 1/0.25 lecithin/cholesterol or stearic acid ratio were effective in protecting enzyme against acid (pH 2.8) and pepsin (15 mg/mL). Use of stearic acid, instead of cholesterol, would be feasible in preparing stable liposomes.
1750-3841
17503841
shingle_title_1 Properties of Liposomes Prepared with Various Lipids
shingle_title_2 Properties of Liposomes Prepared with Various Lipids
shingle_title_3 Properties of Liposomes Prepared with Various Lipids
shingle_title_4 Properties of Liposomes Prepared with Various Lipids
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titel Properties of Liposomes Prepared with Various Lipids
titel_suche Properties of Liposomes Prepared with Various Lipids
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