Expression of midkine and receptor-like protein tyrosine phosphatase (RPTP)-β genes in the rat stomach and the influence of rebamipide
Yuki, T. ; Ishihara, S. ; Rumi, M. ; Ortega-Cava Cesar, F. ; Kadowaki, Y. ; Kazumori, H. ; Yuki, M. ; Wada, T. ; Miyaoka, Y. ; Yoshino, N. ; Kinoshita, Y.
Oxford, UK : Blackwell Publishing Ltd
Published 2003
Oxford, UK : Blackwell Publishing Ltd
Published 2003
| ISSN: |
1365-2036
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Background : Midkine has been reported to bind to receptor-like protein tyrosine phosphatase (RPTP)-β and to play important roles in growth and differentiation of various cells. Midkine is expressed in rat stomach during experimental ulcer healing, suggesting that the midkine-RPTP-β system has some physiological functions in the stomach. Rebamipide is a mucoprotective drug used for the treatment of gastric ulcers. We have tested the hypothesis that the ulcer healing mechanism stimulated by rebamipide is linked physiologically to the gastric midkine-RPTP-β system.Materials and methods : Seven-week-old-male Wistar rats were used. Midkine and RPTP-β gene expression in rat stomach was investigated by laser capture microdissection coupled with the reverse transcription-polymerase chain reaction (RT-PCR). The effects of rebamipide on midkine and RPTP-β expression in rat stomach and the gastric epithelial cell line RGM1 were evaluated by RT-PCR and Northern blot analyses.Results : Midkine and RPTP-β expression was detected in the gastric mucosal, submucosal and muscle layers. Rebamipide stimulated both midkine and RPTP-β expression in rat stomach and RGM1 cells.Conclusion : Rebamipide may protect the gastric mucosa by regulating midkine and RPTP-β expression.
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| Type of Medium: |
Electronic Resource
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| URL: |