Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells

FAURE, M. ; DEZUTTER-DAMBUYANT, C. ; SCHMITT, D. ; GAUCHERAND, M. ; THIVOLET, J.

Oxford, UK : Blackwell Publishing Ltd
Published 1985
ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Human epidermal cells act as stimulators in the mixed-skin cell lymphocyte culture reaction (MSLR). To analyse the generation in MSLR of alloreactive cytotoxic T lymphocytes (CTL) in cell-mediated cytolysis of human epidermal cells, a phenomenon suggested by various observations of skin inflammatory processes in vivo, 18-h 51Cr-release assays against epidermal cells cultivated on collagen-coated plates (epidermal cells autologous to the stimulator cells in MSLR) were conducted after allogenic human MSLR. To analyse the role of human Langerhans cells and related epidermal dendritic cells, which are the only cells expressing the DR-Ia-like (class II) antigens in normal epidermis and in suspensions of normal epidermal cells, MSLR and CTL assays were conducted with, as stimulator cells, suspensions of normal human epidermal cells as controls, and, in parallel, suspensions of epidermal cells after preincubation with anti-class II monoclonal antibody and complement. The generation of alloreactive CTL to epidermal cells occurred only after allogenic MSLR and when targets and stimulator cells were from the same donor; it was abolished when epidermal cell suspensions used in MSLR were depleted in HLA-DR-expressing cells. These findings demonstrate that an epidermal cell-induced generation of cell-mediated cytotoxicity to human epidermal cells may occur in vitro. Langerhans cells and other class II antigen-expressing epidermal cells (dendritic indeterminate cells) are necessary for an optimal in vitro sensitization in MSLR and the subsequent generation of alloreactive CTL towards epidermal cells in man.
Type of Medium:
Electronic Resource
URL:
_version_ 1798290238846533633
autor FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
autorsonst GAUCHERAND, M.
THIVOLET, J.
book_url http://dx.doi.org/10.1111/j.1365-3083.1985.tb01830.x
datenlieferant nat_lic_papers
hauptsatz hsatz_simple
identnr NLZ241966132
insertion_date 2012-04-27
issn 1365-3083
journal_name Scandinavian journal of immunology
materialart 1
notes Human epidermal cells act as stimulators in the mixed-skin cell lymphocyte culture reaction (MSLR). To analyse the generation in MSLR of alloreactive cytotoxic T lymphocytes (CTL) in cell-mediated cytolysis of human epidermal cells, a phenomenon suggested by various observations of skin inflammatory processes in vivo, 18-h 51Cr-release assays against epidermal cells cultivated on collagen-coated plates (epidermal cells autologous to the stimulator cells in MSLR) were conducted after allogenic human MSLR. To analyse the role of human Langerhans cells and related epidermal dendritic cells, which are the only cells expressing the DR-Ia-like (class II) antigens in normal epidermis and in suspensions of normal epidermal cells, MSLR and CTL assays were conducted with, as stimulator cells, suspensions of normal human epidermal cells as controls, and, in parallel, suspensions of epidermal cells after preincubation with anti-class II monoclonal antibody and complement. The generation of alloreactive CTL to epidermal cells occurred only after allogenic MSLR and when targets and stimulator cells were from the same donor; it was abolished when epidermal cell suspensions used in MSLR were depleted in HLA-DR-expressing cells. These findings demonstrate that an epidermal cell-induced generation of cell-mediated cytotoxicity to human epidermal cells may occur in vitro. Langerhans cells and other class II antigen-expressing epidermal cells (dendritic indeterminate cells) are necessary for an optimal in vitro sensitization in MSLR and the subsequent generation of alloreactive CTL towards epidermal cells in man.
package_name Blackwell Publishing
publikationsjahr_anzeige 1985
publikationsjahr_facette 1985
publikationsjahr_intervall 8014:1985-1989
publikationsjahr_sort 1985
publikationsort Oxford, UK
publisher Blackwell Publishing Ltd
reference 21 (1985), S. 0
search_space articles
shingle_author_1 FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
shingle_author_2 FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
shingle_author_3 FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
shingle_author_4 FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
shingle_catch_all_1 FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
Blackwell Publishing Ltd
Human epidermal cells act as stimulators in the mixed-skin cell lymphocyte culture reaction (MSLR). To analyse the generation in MSLR of alloreactive cytotoxic T lymphocytes (CTL) in cell-mediated cytolysis of human epidermal cells, a phenomenon suggested by various observations of skin inflammatory processes in vivo, 18-h 51Cr-release assays against epidermal cells cultivated on collagen-coated plates (epidermal cells autologous to the stimulator cells in MSLR) were conducted after allogenic human MSLR. To analyse the role of human Langerhans cells and related epidermal dendritic cells, which are the only cells expressing the DR-Ia-like (class II) antigens in normal epidermis and in suspensions of normal epidermal cells, MSLR and CTL assays were conducted with, as stimulator cells, suspensions of normal human epidermal cells as controls, and, in parallel, suspensions of epidermal cells after preincubation with anti-class II monoclonal antibody and complement. The generation of alloreactive CTL to epidermal cells occurred only after allogenic MSLR and when targets and stimulator cells were from the same donor; it was abolished when epidermal cell suspensions used in MSLR were depleted in HLA-DR-expressing cells. These findings demonstrate that an epidermal cell-induced generation of cell-mediated cytotoxicity to human epidermal cells may occur in vitro. Langerhans cells and other class II antigen-expressing epidermal cells (dendritic indeterminate cells) are necessary for an optimal in vitro sensitization in MSLR and the subsequent generation of alloreactive CTL towards epidermal cells in man.
1365-3083
13653083
shingle_catch_all_2 FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
Blackwell Publishing Ltd
Human epidermal cells act as stimulators in the mixed-skin cell lymphocyte culture reaction (MSLR). To analyse the generation in MSLR of alloreactive cytotoxic T lymphocytes (CTL) in cell-mediated cytolysis of human epidermal cells, a phenomenon suggested by various observations of skin inflammatory processes in vivo, 18-h 51Cr-release assays against epidermal cells cultivated on collagen-coated plates (epidermal cells autologous to the stimulator cells in MSLR) were conducted after allogenic human MSLR. To analyse the role of human Langerhans cells and related epidermal dendritic cells, which are the only cells expressing the DR-Ia-like (class II) antigens in normal epidermis and in suspensions of normal epidermal cells, MSLR and CTL assays were conducted with, as stimulator cells, suspensions of normal human epidermal cells as controls, and, in parallel, suspensions of epidermal cells after preincubation with anti-class II monoclonal antibody and complement. The generation of alloreactive CTL to epidermal cells occurred only after allogenic MSLR and when targets and stimulator cells were from the same donor; it was abolished when epidermal cell suspensions used in MSLR were depleted in HLA-DR-expressing cells. These findings demonstrate that an epidermal cell-induced generation of cell-mediated cytotoxicity to human epidermal cells may occur in vitro. Langerhans cells and other class II antigen-expressing epidermal cells (dendritic indeterminate cells) are necessary for an optimal in vitro sensitization in MSLR and the subsequent generation of alloreactive CTL towards epidermal cells in man.
1365-3083
13653083
shingle_catch_all_3 FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
Blackwell Publishing Ltd
Human epidermal cells act as stimulators in the mixed-skin cell lymphocyte culture reaction (MSLR). To analyse the generation in MSLR of alloreactive cytotoxic T lymphocytes (CTL) in cell-mediated cytolysis of human epidermal cells, a phenomenon suggested by various observations of skin inflammatory processes in vivo, 18-h 51Cr-release assays against epidermal cells cultivated on collagen-coated plates (epidermal cells autologous to the stimulator cells in MSLR) were conducted after allogenic human MSLR. To analyse the role of human Langerhans cells and related epidermal dendritic cells, which are the only cells expressing the DR-Ia-like (class II) antigens in normal epidermis and in suspensions of normal epidermal cells, MSLR and CTL assays were conducted with, as stimulator cells, suspensions of normal human epidermal cells as controls, and, in parallel, suspensions of epidermal cells after preincubation with anti-class II monoclonal antibody and complement. The generation of alloreactive CTL to epidermal cells occurred only after allogenic MSLR and when targets and stimulator cells were from the same donor; it was abolished when epidermal cell suspensions used in MSLR were depleted in HLA-DR-expressing cells. These findings demonstrate that an epidermal cell-induced generation of cell-mediated cytotoxicity to human epidermal cells may occur in vitro. Langerhans cells and other class II antigen-expressing epidermal cells (dendritic indeterminate cells) are necessary for an optimal in vitro sensitization in MSLR and the subsequent generation of alloreactive CTL towards epidermal cells in man.
1365-3083
13653083
shingle_catch_all_4 FAURE, M.
DEZUTTER-DAMBUYANT, C.
SCHMITT, D.
GAUCHERAND, M.
THIVOLET, J.
Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
Blackwell Publishing Ltd
Human epidermal cells act as stimulators in the mixed-skin cell lymphocyte culture reaction (MSLR). To analyse the generation in MSLR of alloreactive cytotoxic T lymphocytes (CTL) in cell-mediated cytolysis of human epidermal cells, a phenomenon suggested by various observations of skin inflammatory processes in vivo, 18-h 51Cr-release assays against epidermal cells cultivated on collagen-coated plates (epidermal cells autologous to the stimulator cells in MSLR) were conducted after allogenic human MSLR. To analyse the role of human Langerhans cells and related epidermal dendritic cells, which are the only cells expressing the DR-Ia-like (class II) antigens in normal epidermis and in suspensions of normal epidermal cells, MSLR and CTL assays were conducted with, as stimulator cells, suspensions of normal human epidermal cells as controls, and, in parallel, suspensions of epidermal cells after preincubation with anti-class II monoclonal antibody and complement. The generation of alloreactive CTL to epidermal cells occurred only after allogenic MSLR and when targets and stimulator cells were from the same donor; it was abolished when epidermal cell suspensions used in MSLR were depleted in HLA-DR-expressing cells. These findings demonstrate that an epidermal cell-induced generation of cell-mediated cytotoxicity to human epidermal cells may occur in vitro. Langerhans cells and other class II antigen-expressing epidermal cells (dendritic indeterminate cells) are necessary for an optimal in vitro sensitization in MSLR and the subsequent generation of alloreactive CTL towards epidermal cells in man.
1365-3083
13653083
shingle_title_1 Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
shingle_title_2 Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
shingle_title_3 Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
shingle_title_4 Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
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wilbert
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source_archive Blackwell Publishing Journal Backfiles 1879-2005
timestamp 2024-05-06T08:13:33.854Z
titel Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
titel_suche Human Epidermal Cell-Induced Generation of Alloreactive Cytotoxic T-Lymphocyte Responses against Epidermal Cells
topic WW-YZ
uid nat_lic_papers_NLZ241966132