Isolation of α-tubulin genes from the human malaria parasite, Plasmodium falciparum: sequence analysis of α-tubulin
Holloway, S. P. ; Sims, P. F. G. ; Delves, C. J. ; Scaife, J. G. ; Hyde, J. E.
Oxford, UK : Blackwell Publishing Ltd
Published 1989
Oxford, UK : Blackwell Publishing Ltd
Published 1989
| ISSN: |
1365-2958
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Biology
Medicine
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| Notes: |
As a step towards indentifying exploitable differences between host and parasite at the molecular level, we have isolated and sequenced genomic clones encompassing an entire α-tubulin gene (designated α-tubulin I) from the human malaria parasite, Plasmodium falciparum. The gene, which contains two introns, encodes a product with a predicted length of 453 amino acid residues (50.3 kD). The protein sequence shows a high degree of homology to other α-tubulins, particularly that of the coccidian parasite, Toxoplasma gondii (94%), whose gene carries introns in identical positions. Only one copy of the α-tubulin I gene itself was found, although a second gene designated α-II was also identified which is closely related but which differs at both the nucleotide and amino acid sequence levels. The α-I and β-tubulin genes were found to reside on different chromosomes.
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| Type of Medium: |
Electronic Resource
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| URL: |