Molecular cloning and characterization of chromosomal virulence region kcpA of Shigella flexneri
Yamada, M. ; Sasakawa, C. ; Okada, N. ; Makino, S.-I. ; Yoshikawa, M.
Oxford, UK : Blackwell Publishing Ltd
Published 1989
Oxford, UK : Blackwell Publishing Ltd
Published 1989
| ISSN: |
1365-2958
|
|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
|
| Topics: |
Biology
Medicine
|
| Notes: |
In Shigella flexneri, in addition to several well-recognized plasmid-borne virulence loci, at least three genetic loci implicated in pathogenesis have been recognized on the chromosome. To understand more about the pathogenesis of bacillary dysentery at a molecular level, the genetically recognized but previously unidentified KcpA region (one of the chromosomal regions near purE) was cloned and sequenced. A single translatable open reading frame encoding a 12310 Dalton protein corresponding to the minicell product was found. Immunofluorescence microscopy, as well as optical and electron microscopic comparison of tissue-cultured cells and guinea-pigs’eyes infected with wild-type or kcpA mutant bacteria, revealed that the kcpA product is required by invading bacteria for spread into adjacent cells.
|
| Type of Medium: |
Electronic Resource
|
| URL: |