Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a
Sasakawa, C. ; Adler, B. ; Tobe, T. ; Okada, N. ; Nagai, S. ; Komatsu, K. ; Yoshikawa, M.
Oxford, UK : Blackwell Publishing Ltd
Published 1989
Oxford, UK : Blackwell Publishing Ltd
Published 1989
| ISSN: |
1365-2958
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|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Biology
Medicine
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| Notes: |
The 7kb virulence Region-2 of the large (virulence) plasmid in Shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. Insertion and deletion mutagenesis, DNA subcloning and SDS-polyacrylamide gel electro-phoresis of proteins synthesized in minicells demonstrated five genes in this region. They encode 24, 18, 62 (lpaB), 41 (lpaC) and 37 (lpaD)-kiloDalton (kD) proteins. Complementation of Tn5-induced mutations in Region-2 with the above plasmid constructs indicated that Region-2 consists of two operons and that the three lpa proteins are essential for the virulence phenotype. The transcriptional organization determined by Northern blotting, S1 nuclease protection and the effect of Tn5 insertions on expression of the lpa proteins revealed that Region-2 has three promoters that transcribe RNAs of 4.0, 4.5 and 7.5kb. The 4.0 kb RNA was the transcript for the operon encoding the 24, 18 kD, lpaB and C proteins and the 4.5 kb RNA for the ipsD gene. In addition, the full-length RNA of 7.5 kb which covers Region-2 supplemented full expression of the lpa proteins. The 7663 nucleotides of Region-2 were determined to confirm the five open reading frames encoding 23655, 17755, 62168, 41077 and 36660 Dalton proteins, respectively, and their regulatory sequences.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798290415794782208 |
|---|---|
| autor | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. |
| autorsonst | Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. |
| book_url | http://dx.doi.org/10.1111/j.1365-2958.1989.tb00269.x |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLZ241779170 |
| insertion_date | 2012-04-27 |
| issn | 1365-2958 |
| journal_name | Molecular microbiology |
| materialart | 1 |
| notes | The 7kb virulence Region-2 of the large (virulence) plasmid in Shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. Insertion and deletion mutagenesis, DNA subcloning and SDS-polyacrylamide gel electro-phoresis of proteins synthesized in minicells demonstrated five genes in this region. They encode 24, 18, 62 (lpaB), 41 (lpaC) and 37 (lpaD)-kiloDalton (kD) proteins. Complementation of Tn5-induced mutations in Region-2 with the above plasmid constructs indicated that Region-2 consists of two operons and that the three lpa proteins are essential for the virulence phenotype. The transcriptional organization determined by Northern blotting, S1 nuclease protection and the effect of Tn5 insertions on expression of the lpa proteins revealed that Region-2 has three promoters that transcribe RNAs of 4.0, 4.5 and 7.5kb. The 4.0 kb RNA was the transcript for the operon encoding the 24, 18 kD, lpaB and C proteins and the 4.5 kb RNA for the ipsD gene. In addition, the full-length RNA of 7.5 kb which covers Region-2 supplemented full expression of the lpa proteins. The 7663 nucleotides of Region-2 were determined to confirm the five open reading frames encoding 23655, 17755, 62168, 41077 and 36660 Dalton proteins, respectively, and their regulatory sequences. |
| package_name | Blackwell Publishing |
| publikationsjahr_anzeige | 1989 |
| publikationsjahr_facette | 1989 |
| publikationsjahr_intervall | 8014:1985-1989 |
| publikationsjahr_sort | 1989 |
| publikationsort | Oxford, UK |
| publisher | Blackwell Publishing Ltd |
| reference | 3 (1989), S. 0 |
| search_space | articles |
| shingle_author_1 | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. |
| shingle_author_2 | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. |
| shingle_author_3 | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. |
| shingle_author_4 | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. |
| shingle_catch_all_1 | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a Blackwell Publishing Ltd The 7kb virulence Region-2 of the large (virulence) plasmid in Shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. Insertion and deletion mutagenesis, DNA subcloning and SDS-polyacrylamide gel electro-phoresis of proteins synthesized in minicells demonstrated five genes in this region. They encode 24, 18, 62 (lpaB), 41 (lpaC) and 37 (lpaD)-kiloDalton (kD) proteins. Complementation of Tn5-induced mutations in Region-2 with the above plasmid constructs indicated that Region-2 consists of two operons and that the three lpa proteins are essential for the virulence phenotype. The transcriptional organization determined by Northern blotting, S1 nuclease protection and the effect of Tn5 insertions on expression of the lpa proteins revealed that Region-2 has three promoters that transcribe RNAs of 4.0, 4.5 and 7.5kb. The 4.0 kb RNA was the transcript for the operon encoding the 24, 18 kD, lpaB and C proteins and the 4.5 kb RNA for the ipsD gene. In addition, the full-length RNA of 7.5 kb which covers Region-2 supplemented full expression of the lpa proteins. The 7663 nucleotides of Region-2 were determined to confirm the five open reading frames encoding 23655, 17755, 62168, 41077 and 36660 Dalton proteins, respectively, and their regulatory sequences. 1365-2958 13652958 |
| shingle_catch_all_2 | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a Blackwell Publishing Ltd The 7kb virulence Region-2 of the large (virulence) plasmid in Shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. Insertion and deletion mutagenesis, DNA subcloning and SDS-polyacrylamide gel electro-phoresis of proteins synthesized in minicells demonstrated five genes in this region. They encode 24, 18, 62 (lpaB), 41 (lpaC) and 37 (lpaD)-kiloDalton (kD) proteins. Complementation of Tn5-induced mutations in Region-2 with the above plasmid constructs indicated that Region-2 consists of two operons and that the three lpa proteins are essential for the virulence phenotype. The transcriptional organization determined by Northern blotting, S1 nuclease protection and the effect of Tn5 insertions on expression of the lpa proteins revealed that Region-2 has three promoters that transcribe RNAs of 4.0, 4.5 and 7.5kb. The 4.0 kb RNA was the transcript for the operon encoding the 24, 18 kD, lpaB and C proteins and the 4.5 kb RNA for the ipsD gene. In addition, the full-length RNA of 7.5 kb which covers Region-2 supplemented full expression of the lpa proteins. The 7663 nucleotides of Region-2 were determined to confirm the five open reading frames encoding 23655, 17755, 62168, 41077 and 36660 Dalton proteins, respectively, and their regulatory sequences. 1365-2958 13652958 |
| shingle_catch_all_3 | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a Blackwell Publishing Ltd The 7kb virulence Region-2 of the large (virulence) plasmid in Shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. Insertion and deletion mutagenesis, DNA subcloning and SDS-polyacrylamide gel electro-phoresis of proteins synthesized in minicells demonstrated five genes in this region. They encode 24, 18, 62 (lpaB), 41 (lpaC) and 37 (lpaD)-kiloDalton (kD) proteins. Complementation of Tn5-induced mutations in Region-2 with the above plasmid constructs indicated that Region-2 consists of two operons and that the three lpa proteins are essential for the virulence phenotype. The transcriptional organization determined by Northern blotting, S1 nuclease protection and the effect of Tn5 insertions on expression of the lpa proteins revealed that Region-2 has three promoters that transcribe RNAs of 4.0, 4.5 and 7.5kb. The 4.0 kb RNA was the transcript for the operon encoding the 24, 18 kD, lpaB and C proteins and the 4.5 kb RNA for the ipsD gene. In addition, the full-length RNA of 7.5 kb which covers Region-2 supplemented full expression of the lpa proteins. The 7663 nucleotides of Region-2 were determined to confirm the five open reading frames encoding 23655, 17755, 62168, 41077 and 36660 Dalton proteins, respectively, and their regulatory sequences. 1365-2958 13652958 |
| shingle_catch_all_4 | Sasakawa, C. Adler, B. Tobe, T. Okada, N. Nagai, S. Komatsu, K. Yoshikawa, M. Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a Blackwell Publishing Ltd The 7kb virulence Region-2 of the large (virulence) plasmid in Shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. Insertion and deletion mutagenesis, DNA subcloning and SDS-polyacrylamide gel electro-phoresis of proteins synthesized in minicells demonstrated five genes in this region. They encode 24, 18, 62 (lpaB), 41 (lpaC) and 37 (lpaD)-kiloDalton (kD) proteins. Complementation of Tn5-induced mutations in Region-2 with the above plasmid constructs indicated that Region-2 consists of two operons and that the three lpa proteins are essential for the virulence phenotype. The transcriptional organization determined by Northern blotting, S1 nuclease protection and the effect of Tn5 insertions on expression of the lpa proteins revealed that Region-2 has three promoters that transcribe RNAs of 4.0, 4.5 and 7.5kb. The 4.0 kb RNA was the transcript for the operon encoding the 24, 18 kD, lpaB and C proteins and the 4.5 kb RNA for the ipsD gene. In addition, the full-length RNA of 7.5 kb which covers Region-2 supplemented full expression of the lpa proteins. The 7663 nucleotides of Region-2 were determined to confirm the five open reading frames encoding 23655, 17755, 62168, 41077 and 36660 Dalton proteins, respectively, and their regulatory sequences. 1365-2958 13652958 |
| shingle_title_1 | Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a |
| shingle_title_2 | Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a |
| shingle_title_3 | Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a |
| shingle_title_4 | Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a |
| sigel_instance_filter | dkfz geomar wilbert ipn albert |
| source_archive | Blackwell Publishing Journal Backfiles 1879-2005 |
| timestamp | 2024-05-06T08:16:22.145Z |
| titel | Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a |
| titel_suche | Functional organization and nucleotide sequence of virulence Region-2 on the large virulence plasmid in Shigella flexneri 2a |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLZ241779170 |