Nodulation in Sesbania aculeata as Affected by Some Pyrimidine Bases and Their Analogues

1399-3054

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Effects of uracil, thymine and their analogues, 5-nitrouracil, 2-thiouracil, 6-azauracil, and 6-azathymine on nodulation in Sesbania aculeata have been studied. The seeds were dipped partially in a 15 ml solution of each of different concentrations. The experiments included treatments for 6 or 12 h with distilled water as control. The treated seeds were inoculated with a pure culture of Rhizobium leguminosarum and sown in sand cultures, weekly supplied with modified White's basic N-free solution.Nodulation in Sesbania aculeata was promoted by uracil, nitrouracil and thymine, but inhibited by analogues. Higher concentrations caused greater inhibition than lower ones. Nodulation was affected by changes in the nucleic acid and protein metabolisms.

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