ISOLATION OF MYELIN FIGURES AND LOW-DENSITY LIPOPROTEINS FROM EGG YOLK GRANULES

1750-3841

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Process Engineering, Biotechnology, Nutrition Technology

The granules of egg yolk were disrupted with 1.71M sodium chloride and separated into two floating and three sedimenting fractions on ultracentrifugation. The pellicle (FI) and subpellicle (FII) fractions contained most of the granular low-density material. A minor amount of low-density lipoproteins was trapped physically within the high-density matrix of sedimenting fractions. The subpellicle (FII) was particularly rich in myelin figures (MF) and uniformly-sized granule low-density lipoproteins (LDLg). Ultrafiltration, dextran sulfate-lipoprotein complexing and gel filtration techniques were evaluated for their ability to separate these two FII components in the purified form. Electron microscopy was used to assess fraction purity and the structural integrity of the particles. Sepharose 2B gel filtration was considered the best method of isolation.

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