An Exchange Assay for the Measurement of Cell Nuclear Estrogen Receptors in Microdissected Brain Regions
Kranzler, J. D. ; Jones, E. E. ; MacLusky, N. J. ; Sakamoto, H. ; Naftolin, F.
Oxford, UK : Blackwell Publishing Ltd
Published 1984
Oxford, UK : Blackwell Publishing Ltd
Published 1984
| ISSN: |
1471-4159
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Abstract: An exchange assay is described for the measurement of nuclear estrogen receptors (ERn) in micro-dissected brain regions. The distribution of ERn in the hypothalamus and amygdala of the rat 1 h after an injection of estradiol (E) is presented. Combining the exchange assay with a previously described method for measurement of cytosol estrogen receptors (ERc) in microdissected brain samples, gonadectomized male and female rats were compared for ERc and ERn. While ERc concentrations tended to be higher in females than in males in all regions of the hypothalamus, with a significant sex difference in the arcuate-median eminence, no sex difference in ERn concentrations was observed after E injection. These results suggest that ERc measurements alone are not sufficient to establish the capacity of the E receptor system: ERn measurements are also necessary to establish the relationship between receptor levels and physiologic estrogen responsiveness.
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| Type of Medium: |
Electronic Resource
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| URL: |