Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis
Vulto, Arnold G. ; Sharp, Trevor ; Ungerstedt, Urban ; Versteeg, Dirk H. G.
Oxford, UK : Blackwell Publishing Ltd
Published 1988
Oxford, UK : Blackwell Publishing Ltd
Published 1988
| ISSN: |
1471-4159
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|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Abstract: Extracellular dopamine (DA) and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanil-lic acid (HVA) in rat nucleus accumbens were determined before and shortly following death using microdialysis. A maximal 400-fold increase in the output of DA was observed within the first 5 min of death. DA output remained elevated over the following hour at a level of approximately 70-fold above pre-death values. In contrast to that of DA, DOPAC and HVA output gradually declined. Before death the extracellular DOPAC/DA ratio was about 250; after death this ratio dropped to 0.44 at 5 min. These observations may have important implications for experiments measuring the output of (endogenous) DA and its metabolites from brain tissue in vitro: autoregulation of, e.g., transmitter release and synthesis in vitro may be seriously disrupted by the observed depletion of transmitter storage granules.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798289989159616512 |
|---|---|
| autor | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. |
| autorsonst | Versteeg, Dirk H. G. |
| book_url | http://dx.doi.org/10.1111/j.1471-4159.1988.tb01808.x |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLZ240279654 |
| insertion_date | 2012-04-26 |
| issn | 1471-4159 |
| journal_name | Journal of neurochemistry |
| materialart | 1 |
| notes | Abstract: Extracellular dopamine (DA) and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanil-lic acid (HVA) in rat nucleus accumbens were determined before and shortly following death using microdialysis. A maximal 400-fold increase in the output of DA was observed within the first 5 min of death. DA output remained elevated over the following hour at a level of approximately 70-fold above pre-death values. In contrast to that of DA, DOPAC and HVA output gradually declined. Before death the extracellular DOPAC/DA ratio was about 250; after death this ratio dropped to 0.44 at 5 min. These observations may have important implications for experiments measuring the output of (endogenous) DA and its metabolites from brain tissue in vitro: autoregulation of, e.g., transmitter release and synthesis in vitro may be seriously disrupted by the observed depletion of transmitter storage granules. |
| package_name | Blackwell Publishing |
| publikationsjahr_anzeige | 1988 |
| publikationsjahr_facette | 1988 |
| publikationsjahr_intervall | 8014:1985-1989 |
| publikationsjahr_sort | 1988 |
| publikationsort | Oxford, UK |
| publisher | Blackwell Publishing Ltd |
| reference | 51 (1988), S. 0 |
| search_space | articles |
| shingle_author_1 | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. |
| shingle_author_2 | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. |
| shingle_author_3 | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. |
| shingle_author_4 | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. |
| shingle_catch_all_1 | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis Blackwell Publishing Ltd Abstract: Extracellular dopamine (DA) and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanil-lic acid (HVA) in rat nucleus accumbens were determined before and shortly following death using microdialysis. A maximal 400-fold increase in the output of DA was observed within the first 5 min of death. DA output remained elevated over the following hour at a level of approximately 70-fold above pre-death values. In contrast to that of DA, DOPAC and HVA output gradually declined. Before death the extracellular DOPAC/DA ratio was about 250; after death this ratio dropped to 0.44 at 5 min. These observations may have important implications for experiments measuring the output of (endogenous) DA and its metabolites from brain tissue in vitro: autoregulation of, e.g., transmitter release and synthesis in vitro may be seriously disrupted by the observed depletion of transmitter storage granules. 1471-4159 14714159 |
| shingle_catch_all_2 | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis Blackwell Publishing Ltd Abstract: Extracellular dopamine (DA) and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanil-lic acid (HVA) in rat nucleus accumbens were determined before and shortly following death using microdialysis. A maximal 400-fold increase in the output of DA was observed within the first 5 min of death. DA output remained elevated over the following hour at a level of approximately 70-fold above pre-death values. In contrast to that of DA, DOPAC and HVA output gradually declined. Before death the extracellular DOPAC/DA ratio was about 250; after death this ratio dropped to 0.44 at 5 min. These observations may have important implications for experiments measuring the output of (endogenous) DA and its metabolites from brain tissue in vitro: autoregulation of, e.g., transmitter release and synthesis in vitro may be seriously disrupted by the observed depletion of transmitter storage granules. 1471-4159 14714159 |
| shingle_catch_all_3 | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis Blackwell Publishing Ltd Abstract: Extracellular dopamine (DA) and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanil-lic acid (HVA) in rat nucleus accumbens were determined before and shortly following death using microdialysis. A maximal 400-fold increase in the output of DA was observed within the first 5 min of death. DA output remained elevated over the following hour at a level of approximately 70-fold above pre-death values. In contrast to that of DA, DOPAC and HVA output gradually declined. Before death the extracellular DOPAC/DA ratio was about 250; after death this ratio dropped to 0.44 at 5 min. These observations may have important implications for experiments measuring the output of (endogenous) DA and its metabolites from brain tissue in vitro: autoregulation of, e.g., transmitter release and synthesis in vitro may be seriously disrupted by the observed depletion of transmitter storage granules. 1471-4159 14714159 |
| shingle_catch_all_4 | Vulto, Arnold G. Sharp, Trevor Ungerstedt, Urban Versteeg, Dirk H. G. Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis Blackwell Publishing Ltd Abstract: Extracellular dopamine (DA) and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanil-lic acid (HVA) in rat nucleus accumbens were determined before and shortly following death using microdialysis. A maximal 400-fold increase in the output of DA was observed within the first 5 min of death. DA output remained elevated over the following hour at a level of approximately 70-fold above pre-death values. In contrast to that of DA, DOPAC and HVA output gradually declined. Before death the extracellular DOPAC/DA ratio was about 250; after death this ratio dropped to 0.44 at 5 min. These observations may have important implications for experiments measuring the output of (endogenous) DA and its metabolites from brain tissue in vitro: autoregulation of, e.g., transmitter release and synthesis in vitro may be seriously disrupted by the observed depletion of transmitter storage granules. 1471-4159 14714159 |
| shingle_title_1 | Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis |
| shingle_title_2 | Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis |
| shingle_title_3 | Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis |
| shingle_title_4 | Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis |
| sigel_instance_filter | dkfz geomar wilbert ipn albert |
| source_archive | Blackwell Publishing Journal Backfiles 1879-2005 |
| timestamp | 2024-05-06T08:09:36.040Z |
| titel | Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis |
| titel_suche | Rapid Postmortem Increase in Extracellular Dopamine in the Rat Brain as Assessed by Brain Microdialysis |
| topic | WW-YZ |
| uid | nat_lic_papers_NLZ240279654 |