Characterization of the Substance P (NK-1) Receptor in Tunicamycin-Treated Transfected Cells Using a Photoaffinity Analogue of Substance P
Kage, R. ; Hershey, A. D. ; Krause, J. E. ; Boyd, N. D. ; Leeman, S. E.
Oxford, UK : Blackwell Science Ltd
Published 1995
Oxford, UK : Blackwell Science Ltd
Published 1995
| ISSN: |
1471-4159
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Abstract: Chinese hamster ovary cells expressing the N-glycosylated substance P (NK-1) receptor were treated with the glycosylation inhibitor tunicamycin and photolabeled with 125I-Bolton-Hunter-p-benzoyl-l-phenylalanine8-substance P. Two radioactive proteins of Mr 80,000 and 46,000, representing the glycosylated and nonglycosylated substance P (NK-1) receptor, respectively, were observed. The IC50 for the inhibition of photolabeling of both receptor forms was 0.3 ± 0.1 nM for substance P and 30 ± 5 nM for neurokinin A (substance K). Thus, glycosylation of the substance P (NK-1) receptor has no detectable effect on the affinity of the substance P (NK-1) receptor for substance P or neurokinin A (substance K).
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| Type of Medium: |
Electronic Resource
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| URL: |