An in vitro study of neutrophils obtained from the normal gingival sulcus
Charon, Jacques A. ; Metzger, Zvi ; Terrell Hoffeld, J. ; Oliver, Contance ; Gallin, John I. ; Mergenhagen, Stephan E.
Oxford, UK : Blackwell Publishing Ltd
Published 1982
Oxford, UK : Blackwell Publishing Ltd
Published 1982
| ISSN: |
1600-0765
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|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Neutrophils (PMN) are considered to be key components in the protection of the periodontium against pathogenic bacteria. We therefore compared five of the major characteristics of peripheral blood PMN (PB-PMN) (adherence, chemotaxis, phagocytosis, oxidaiive metabolism, and lysosomal granules) to those of normal crevicular neutrophils (CR-PMN) isolated from the same individuals. The data indicate that the presence of Fc and C3b receptors, and the production of superoxide are similar in CR-PMN and PB-PMN. In addition, healthy gingival sulci harbor a high percentage of stimulated PMN as determined by the nitroblue tetrazolium (NBT) reduction assay. The percentage of CR-PMN which phagocytized opsonized red blood cells (RBC) was lower than that of PB-PMN. The in vitro migration of CR-PMN was reduced as compared to that of PB-PMN. Crevicular neutrophils also have a diminished ability to adhere to glass surfaces which may be related to a direct non-cytotoxic effect of gingival fluid component(s) on CR-PMN. In addition, morphologic evidence indicates that specific granules are more depleted than azurophil granules in CR-PMN. Interpretation of studies of neutrophil functions in periodontal diseases must consider the observed differences between normal peripheral blood and crevicular neutrophils.
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| Type of Medium: |
Electronic Resource
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| URL: |