Novel polysaccharide capsular serotypes in Porphyromonas gingivalis
Laine, M. L. ; Appelmelk, B. J. ; Winkelhoff, A. J.
Oxford, UK : Blackwell Publishing Ltd
Published 1996
Oxford, UK : Blackwell Publishing Ltd
Published 1996
| ISSN: |
1600-0765
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|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
Recently van Winkelhoff et al. (1) described 3 novel serotypes in virulent Porphyromomas gingivalis strains, which were based on different polysaccharide antigens. These antigens probably represent capsular structures and have been designated K1, K2 and K3. In the present study we report on 3 novel capsular serotypes, which are represented by P. gingivalis strains ATCC 49417, HG 1690 and HG 1691. The strains, isolated from patients with periodontitis, showed obvious encapsulation in wet India ink preparations. Thermostable antigens could be detected in the supernatant fractions of autoclaved cells. These antigens appeared to be negatively charged, sensitive to periodate degradation, and resistant to proteinase K treatment. On the basis of these characteristics we conclude that the antigens are probably extra-cellular polysaccharides representing a bacterial capsular structure. These K-antigens did not cross-react with K1, K2 or K3 immunesera of P. gingivalis, with the exception of the K2 antiserum, which partially recognized K5- and K6-antigens. In contrast, K5 and K6 antisera did not react with the K2-antigen. After absorbtion of the K2 antiserum with cells of strains HG 1690 (K5) and HG 1691 (K6) cross-reactivity was no longer present. We propose these novel serotypes to be designated: K4 (ATCC 49417), K5 (HG 1960) and K6 (HG 1691).
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798290372450844672 |
|---|---|
| autor | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. |
| book_url | http://dx.doi.org/10.1111/j.1600-0765.1996.tb00494.x |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLZ239992458 |
| insertion_date | 2012-04-26 |
| issn | 1600-0765 |
| journal_name | Journal of periodontal research |
| materialart | 1 |
| notes | Recently van Winkelhoff et al. (1) described 3 novel serotypes in virulent Porphyromomas gingivalis strains, which were based on different polysaccharide antigens. These antigens probably represent capsular structures and have been designated K1, K2 and K3. In the present study we report on 3 novel capsular serotypes, which are represented by P. gingivalis strains ATCC 49417, HG 1690 and HG 1691. The strains, isolated from patients with periodontitis, showed obvious encapsulation in wet India ink preparations. Thermostable antigens could be detected in the supernatant fractions of autoclaved cells. These antigens appeared to be negatively charged, sensitive to periodate degradation, and resistant to proteinase K treatment. On the basis of these characteristics we conclude that the antigens are probably extra-cellular polysaccharides representing a bacterial capsular structure. These K-antigens did not cross-react with K1, K2 or K3 immunesera of P. gingivalis, with the exception of the K2 antiserum, which partially recognized K5- and K6-antigens. In contrast, K5 and K6 antisera did not react with the K2-antigen. After absorbtion of the K2 antiserum with cells of strains HG 1690 (K5) and HG 1691 (K6) cross-reactivity was no longer present. We propose these novel serotypes to be designated: K4 (ATCC 49417), K5 (HG 1960) and K6 (HG 1691). |
| package_name | Blackwell Publishing |
| publikationsjahr_anzeige | 1996 |
| publikationsjahr_facette | 1996 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1996 |
| publikationsort | Oxford, UK |
| publisher | Blackwell Publishing Ltd |
| reference | 31 (1996), S. 0 |
| search_space | articles |
| shingle_author_1 | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. |
| shingle_author_2 | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. |
| shingle_author_3 | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. |
| shingle_author_4 | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. |
| shingle_catch_all_1 | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. Novel polysaccharide capsular serotypes in Porphyromonas gingivalis Blackwell Publishing Ltd Recently van Winkelhoff et al. (1) described 3 novel serotypes in virulent Porphyromomas gingivalis strains, which were based on different polysaccharide antigens. These antigens probably represent capsular structures and have been designated K1, K2 and K3. In the present study we report on 3 novel capsular serotypes, which are represented by P. gingivalis strains ATCC 49417, HG 1690 and HG 1691. The strains, isolated from patients with periodontitis, showed obvious encapsulation in wet India ink preparations. Thermostable antigens could be detected in the supernatant fractions of autoclaved cells. These antigens appeared to be negatively charged, sensitive to periodate degradation, and resistant to proteinase K treatment. On the basis of these characteristics we conclude that the antigens are probably extra-cellular polysaccharides representing a bacterial capsular structure. These K-antigens did not cross-react with K1, K2 or K3 immunesera of P. gingivalis, with the exception of the K2 antiserum, which partially recognized K5- and K6-antigens. In contrast, K5 and K6 antisera did not react with the K2-antigen. After absorbtion of the K2 antiserum with cells of strains HG 1690 (K5) and HG 1691 (K6) cross-reactivity was no longer present. We propose these novel serotypes to be designated: K4 (ATCC 49417), K5 (HG 1960) and K6 (HG 1691). 1600-0765 16000765 |
| shingle_catch_all_2 | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. Novel polysaccharide capsular serotypes in Porphyromonas gingivalis Blackwell Publishing Ltd Recently van Winkelhoff et al. (1) described 3 novel serotypes in virulent Porphyromomas gingivalis strains, which were based on different polysaccharide antigens. These antigens probably represent capsular structures and have been designated K1, K2 and K3. In the present study we report on 3 novel capsular serotypes, which are represented by P. gingivalis strains ATCC 49417, HG 1690 and HG 1691. The strains, isolated from patients with periodontitis, showed obvious encapsulation in wet India ink preparations. Thermostable antigens could be detected in the supernatant fractions of autoclaved cells. These antigens appeared to be negatively charged, sensitive to periodate degradation, and resistant to proteinase K treatment. On the basis of these characteristics we conclude that the antigens are probably extra-cellular polysaccharides representing a bacterial capsular structure. These K-antigens did not cross-react with K1, K2 or K3 immunesera of P. gingivalis, with the exception of the K2 antiserum, which partially recognized K5- and K6-antigens. In contrast, K5 and K6 antisera did not react with the K2-antigen. After absorbtion of the K2 antiserum with cells of strains HG 1690 (K5) and HG 1691 (K6) cross-reactivity was no longer present. We propose these novel serotypes to be designated: K4 (ATCC 49417), K5 (HG 1960) and K6 (HG 1691). 1600-0765 16000765 |
| shingle_catch_all_3 | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. Novel polysaccharide capsular serotypes in Porphyromonas gingivalis Blackwell Publishing Ltd Recently van Winkelhoff et al. (1) described 3 novel serotypes in virulent Porphyromomas gingivalis strains, which were based on different polysaccharide antigens. These antigens probably represent capsular structures and have been designated K1, K2 and K3. In the present study we report on 3 novel capsular serotypes, which are represented by P. gingivalis strains ATCC 49417, HG 1690 and HG 1691. The strains, isolated from patients with periodontitis, showed obvious encapsulation in wet India ink preparations. Thermostable antigens could be detected in the supernatant fractions of autoclaved cells. These antigens appeared to be negatively charged, sensitive to periodate degradation, and resistant to proteinase K treatment. On the basis of these characteristics we conclude that the antigens are probably extra-cellular polysaccharides representing a bacterial capsular structure. These K-antigens did not cross-react with K1, K2 or K3 immunesera of P. gingivalis, with the exception of the K2 antiserum, which partially recognized K5- and K6-antigens. In contrast, K5 and K6 antisera did not react with the K2-antigen. After absorbtion of the K2 antiserum with cells of strains HG 1690 (K5) and HG 1691 (K6) cross-reactivity was no longer present. We propose these novel serotypes to be designated: K4 (ATCC 49417), K5 (HG 1960) and K6 (HG 1691). 1600-0765 16000765 |
| shingle_catch_all_4 | Laine, M. L. Appelmelk, B. J. Winkelhoff, A. J. Novel polysaccharide capsular serotypes in Porphyromonas gingivalis Blackwell Publishing Ltd Recently van Winkelhoff et al. (1) described 3 novel serotypes in virulent Porphyromomas gingivalis strains, which were based on different polysaccharide antigens. These antigens probably represent capsular structures and have been designated K1, K2 and K3. In the present study we report on 3 novel capsular serotypes, which are represented by P. gingivalis strains ATCC 49417, HG 1690 and HG 1691. The strains, isolated from patients with periodontitis, showed obvious encapsulation in wet India ink preparations. Thermostable antigens could be detected in the supernatant fractions of autoclaved cells. These antigens appeared to be negatively charged, sensitive to periodate degradation, and resistant to proteinase K treatment. On the basis of these characteristics we conclude that the antigens are probably extra-cellular polysaccharides representing a bacterial capsular structure. These K-antigens did not cross-react with K1, K2 or K3 immunesera of P. gingivalis, with the exception of the K2 antiserum, which partially recognized K5- and K6-antigens. In contrast, K5 and K6 antisera did not react with the K2-antigen. After absorbtion of the K2 antiserum with cells of strains HG 1690 (K5) and HG 1691 (K6) cross-reactivity was no longer present. We propose these novel serotypes to be designated: K4 (ATCC 49417), K5 (HG 1960) and K6 (HG 1691). 1600-0765 16000765 |
| shingle_title_1 | Novel polysaccharide capsular serotypes in Porphyromonas gingivalis |
| shingle_title_2 | Novel polysaccharide capsular serotypes in Porphyromonas gingivalis |
| shingle_title_3 | Novel polysaccharide capsular serotypes in Porphyromonas gingivalis |
| shingle_title_4 | Novel polysaccharide capsular serotypes in Porphyromonas gingivalis |
| sigel_instance_filter | dkfz geomar wilbert ipn albert |
| source_archive | Blackwell Publishing Journal Backfiles 1879-2005 |
| timestamp | 2024-05-06T08:15:40.105Z |
| titel | Novel polysaccharide capsular serotypes in Porphyromonas gingivalis |
| titel_suche | Novel polysaccharide capsular serotypes in Porphyromonas gingivalis |
| topic | WW-YZ |
| uid | nat_lic_papers_NLZ239992458 |