Inhibition of mediator and cytokine release from dispersed nasal polyp cells by terfenadine
Crampette, I. ; Mainprice, B. ; Bloom, M. ; Bousauet, J. ; Campbell, A. M.
Oxford, UK : Blackwell Publishing Ltd
Published 1996
Oxford, UK : Blackwell Publishing Ltd
Published 1996
| ISSN: |
1398-9995
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| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
The mechanism of action of H1-lockers requires elucidation because they may possess properties unrelated to the blockage of histamine at its receptor level. A study was performed with enzymatically dispersed cells obtained from nasal polyps to examine the effect of terfenadine (0.1–10 μmol) on the release of leukotrienes (LT) (LTC4/D4 and LTB4) after stimulation by anti-IgE, and on the spontaneous release of cytokines (granulocyte/macrophage-colony stimulating factor [GM-CSF] and tumor necrosis factor-alpha [TNF-α]) released from cells cultured for 6 h. Terfenadine inhibited significantly, and in a dose-dependent manner, the release of LTC4/D4, LTB4, TNF-α, and GM-CSF. IC50 values were determined for LTC4/D, (8 μmol), LTB4 (9.9 μmol), TNF-α (6.1 μmol), and GM-CSF (4 μmol). Terfenadine was found to possess new antiallergic properties with a novel in vitro model which mimics more closely inflammatory cells of allergic rhinitis or asthma.
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| Type of Medium: |
Electronic Resource
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| URL: |