A new immunoperoxidase assay for Lolium perenne-specltic IgE in serum based on the biotin/avidin system (BAS)
PLEBANI, A. ; NOTARANGELO, L. D. ; MONAFO, V. ; NESPOLI, L. ; UGAZIO, A.G.
Oxford, UK : Blackwell Publishing Ltd
Published 1984
Oxford, UK : Blackwell Publishing Ltd
Published 1984
| ISSN: |
1365-2222
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|---|---|
| Source: |
Blackwell Publishing Journal Backfiles 1879-2005
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| Topics: |
Medicine
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| Notes: |
A new solid-phase immunoassay based on the biotin/avidin system (BAS) for measuring scrum Lolium perenne (LP)-specific IgE antibody is described. LP-specific IgE was assayed by the BAS assay and RAST for comparison in the sera of thirty-two normal asymptomatic subjects R AST-negative for LP and of twenty-six subjects with hay fever and R AST-positive for LP. The specificity of the BAS assay for LP-specific IgE was demonstrated by absorption experiments. An overall agreement of 91% (53/58) was observed between the BAS and RAST and a high correlation (r= 0.87. P 〈 0.001) was found between the LP-specific IgE determined by the two methods. The advantages of ihe BAS assay as compared to both the RAST and classical ELISA arc discussed.
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| Type of Medium: |
Electronic Resource
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| URL: |