Initiation of V(D)J recombination in vitro obeying the 12/23 rule

Eastman, Quinn M. ; Leu, Thomas M. J. ; Schatz, David G.

[s.l.] : Nature Publishing Group
Published 1996
ISSN:
1476-4687
Source:
Nature Archives 1869 - 2009
Topics:
Biology
Chemistry and Pharmacology
Medicine
Natural Sciences in General
Physics
Notes:
[Auszug] The prototype substrate, p!2 x 23 (Fig. 10), is a 531-base-pair (bp) product of the polymerase chain reaction (PCR), uniformly labelled with 32P. It contains a recombination signal with a 12-bp spacer (12-signal) and another with a 23-bp spacer (23-signal). The other substrates used in the initial ...
Type of Medium:
Electronic Resource
URL: