Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging

Hoebe, R A ; Van Oven, C H ; Gadella, T W J ; Dhonukshe, P B ; Van Noorden, C J F ; Manders, E M M

[s.l.] : Nature Publishing Group
Published 2007
ISSN:
1546-1696
Source:
Nature Archives 1869 - 2009
Topics:
Biology
Process Engineering, Biotechnology, Nutrition Technology
Notes:
[Auszug] Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy ...
Type of Medium:
Electronic Resource
URL: