Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line
| ISSN: |
0248-4900
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|---|---|
| Keywords: |
adenocarcinoma cells ; confocal laser scanning microscopy ; intercellular cysts ; intracellular lumens
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| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
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| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798291725558480896 |
|---|---|
| autor | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. |
| autorsonst | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. |
| book_url | http://dx.doi.org/10.1016/0248-4900(90)90339-5 |
| datenlieferant | nat_lic_papers |
| fussnote | Adenocarcinoma cells often form intracellular lumens and intercellular cysts. In order to study the structural relationships between these lumens and the apical domain of normal enterocytes, we have applied electron microscopy and confocal microscopy to a cloned cell line derived from the human colon adenocarcinoma cell line LoVo which express a high number of intracellular lumens and intercellular cysts. Microvilli reminiscent of those detected in the brush border of small intestinal cells are formed in the two types of compartments. By immunofluorescence, we found that a 135 kDa membrane glycoprotein characterized by a monoclonal Ab and normally associated with the brush-border of enterocytes is expressed at the surface of the intracellular lumens and intercellular cysts present in the adenocarcinoma cells. Comparison of fluorescence and reflection contrast micrographs obtained by confocal microscopy demonstrate the presence of spherical intracellular lumens in the juxtanuclear region of single cells, and of more complex shaped intercellular cysts located within clusters of cells. The later cells form junctional complexes limiting an apical plasma membrane domain in contact with the intercellular cyst. It is suggested that the intracellular lumens may represent the abortive form of an apical plasma membrane due to the lack of components required to establish epithelial cell contacts. As opposed to conventional fluorescence microscopy, confocal microscopy allows rapid inspection of the tridimensional organization of intracellular lumens and intercellular cysts even when they are located in cell multilayers. |
| hauptsatz | hsatz_simple |
| identnr | NLZ18786893X |
| issn | 0248-4900 |
| journal_name | Biology of the Cell |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 69 (1990), S. 129-138 |
| schlagwort | adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens |
| search_space | articles |
| shingle_author_1 | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. |
| shingle_author_2 | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. |
| shingle_author_3 | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. |
| shingle_author_4 | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. |
| shingle_catch_all_1 | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens 0248-4900 02484900 Elsevier |
| shingle_catch_all_2 | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens 0248-4900 02484900 Elsevier |
| shingle_catch_all_3 | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens 0248-4900 02484900 Elsevier |
| shingle_catch_all_4 | Remy, L. Gorvel, J.-P. Jacquier, M.-F. Rigal, A. Davoust, J. Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens adenocarcinoma cells confocal laser scanning microscopy intercellular cysts intracellular lumens 0248-4900 02484900 Elsevier |
| shingle_title_1 | Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line |
| shingle_title_2 | Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line |
| shingle_title_3 | Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line |
| shingle_title_4 | Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:37:11.898Z |
| titel | Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line |
| titel_suche | Confocal microscopy as a tool to reveal the tridimensional organization of intracellular lumens and intercellular cysts in a human colon adenocarcinoma cell line Adenocarcinoma cells often form intracellular lumens and intercellular cysts. In order to study the structural relationships between these lumens and the apical domain of normal enterocytes, we have applied electron microscopy and confocal microscopy to a cloned cell line derived from the human colon adenocarcinoma cell line LoVo which express a high number of intracellular lumens and intercellular cysts. Microvilli reminiscent of those detected in the brush border of small intestinal cells are formed in the two types of compartments. By immunofluorescence, we found that a 135 kDa membrane glycoprotein characterized by a monoclonal Ab and normally associated with the brush-border of enterocytes is expressed at the surface of the intracellular lumens and intercellular cysts present in the adenocarcinoma cells. Comparison of fluorescence and reflection contrast micrographs obtained by confocal microscopy demonstrate the presence of spherical intracellular lumens in the juxtanuclear region of single cells, and of more complex shaped intercellular cysts located within clusters of cells. The later cells form junctional complexes limiting an apical plasma membrane domain in contact with the intercellular cyst. It is suggested that the intracellular lumens may represent the abortive form of an apical plasma membrane due to the lack of components required to establish epithelial cell contacts. As opposed to conventional fluorescence microscopy, confocal microscopy allows rapid inspection of the tridimensional organization of intracellular lumens and intercellular cysts even when they are located in cell multilayers. |
| topic | W |
| uid | nat_lic_papers_NLZ18786893X |