Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals
Kato, Y. ; Motoi, Y. ; Taniai, K. ; Kadono-Okuda, K. ; Yamamoto, M. ; Higashino, Y. ; Shimabukuro, M. ; Chowdhury, S. ; Xu, J. ; Hiramatsu, M. ; Sugiyama, M. ; Yamakawa, M.
Amsterdam : Elsevier
Amsterdam : Elsevier
| ISSN: |
0965-1748
|
|---|---|
| Keywords: |
Antibacterial protein ; Cecropin ; Endotoxin ; Insect immunity
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292140579618816 |
|---|---|
| autor | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. |
| autorsonst | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0965-1748(94)90090-6 |
| datenlieferant | nat_lic_papers |
| fussnote | The formation of the lipophorin-lipopolysaccharide (LPS) complex in Bombyx mori hemolymph and its role in LPS detoxification were explored. LPS, an antibacterial protein inducer in insects, was injected into B. mori larvae. Analytical density gradient ultracentrifugation revealed that after injection the LPS peak shifts to a zone of lower density with time. The shifted peak was identified as the lipophorin-LPS complex. This complex formation was also achieved in an in vitro mixture of cell-free hemolymph and LPS at 25^oC but not at 1^oC. The lipophorin-LPS complex had a significantly lower capacity to elicit the mRNA of cecropin B, an antibacterial protein. The biological activity of reextracted LPS from the complex was slightly reduced in the Limulus test and no structural modification was observed in sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These results suggested that the formation of lipophorin-LPS strikingly reduces the cecropin inducibility of LPS without any structural change in LPS. Similar serum lipoprotein-LPS complex formation and reduction of biological activities of LPS were also observed in mammals. We, therefore, suggest that the formation of the serum lipoprotein-LPS complex is a common pathway to inactive LPS both in insects and in mammals. |
| hauptsatz | hsatz_simple |
| identnr | NLZ187431442 |
| issn | 0965-1748 |
| journal_name | Insect Biochemistry and Molecular Biology |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 24 (1994), S. 547-555 |
| schlagwort | Antibacterial protein Cecropin Endotoxin Insect immunity |
| search_space | articles |
| shingle_author_1 | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. |
| shingle_author_2 | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. |
| shingle_author_3 | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. |
| shingle_author_4 | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. |
| shingle_catch_all_1 | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals Antibacterial protein Cecropin Endotoxin Insect immunity Antibacterial protein Cecropin Endotoxin Insect immunity 0965-1748 09651748 Elsevier |
| shingle_catch_all_2 | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals Antibacterial protein Cecropin Endotoxin Insect immunity Antibacterial protein Cecropin Endotoxin Insect immunity 0965-1748 09651748 Elsevier |
| shingle_catch_all_3 | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals Antibacterial protein Cecropin Endotoxin Insect immunity Antibacterial protein Cecropin Endotoxin Insect immunity 0965-1748 09651748 Elsevier |
| shingle_catch_all_4 | Kato, Y. Motoi, Y. Taniai, K. Kadono-Okuda, K. Yamamoto, M. Higashino, Y. Shimabukuro, M. Chowdhury, S. Xu, J. Hiramatsu, M. Sugiyama, M. Yamakawa, M. Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals Antibacterial protein Cecropin Endotoxin Insect immunity Antibacterial protein Cecropin Endotoxin Insect immunity 0965-1748 09651748 Elsevier |
| shingle_title_1 | Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals |
| shingle_title_2 | Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals |
| shingle_title_3 | Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals |
| shingle_title_4 | Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:43:47.169Z |
| titel | Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals |
| titel_suche | Lipopolysaccharide-lipophorin complex formation in insect hemolymph: a common pathway of lipopolysaccharide detoxification both in insects and in mammals The formation of the lipophorin-lipopolysaccharide (LPS) complex in Bombyx mori hemolymph and its role in LPS detoxification were explored. LPS, an antibacterial protein inducer in insects, was injected into B. mori larvae. Analytical density gradient ultracentrifugation revealed that after injection the LPS peak shifts to a zone of lower density with time. The shifted peak was identified as the lipophorin-LPS complex. This complex formation was also achieved in an in vitro mixture of cell-free hemolymph and LPS at 25^oC but not at 1^oC. The lipophorin-LPS complex had a significantly lower capacity to elicit the mRNA of cecropin B, an antibacterial protein. The biological activity of reextracted LPS from the complex was slightly reduced in the Limulus test and no structural modification was observed in sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These results suggested that the formation of lipophorin-LPS strikingly reduces the cecropin inducibility of LPS without any structural change in LPS. Similar serum lipoprotein-LPS complex formation and reduction of biological activities of LPS were also observed in mammals. We, therefore, suggest that the formation of the serum lipoprotein-LPS complex is a common pathway to inactive LPS both in insects and in mammals. |
| topic | W |
| uid | nat_lic_papers_NLZ187431442 |