Detection of oxygen radicals during reperfursion of intestinal cell in vitro
Nilsson, U.A. ; Olsson, L.-I. ; Thor, H. ; Moldeus, P. ; Bylund-Fellenius, A.-C.
Amsterdam : Elsevier
Amsterdam : Elsevier
| ISSN: |
0891-5849
|
|---|---|
| Keywords: |
ESR ; Ischemia and reperfusion ; Nitroxyl radicals ; Small intestine ; Spin trapping ; Superoxide ; Superoxide dismutase
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
Medicine
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292511083462657 |
|---|---|
| autor | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. |
| autorsonst | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0891-5849(89)90052-X |
| datenlieferant | nat_lic_papers |
| fussnote | The nitroxide OXANO. (2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl) which in its reduced form, OXANOH (2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine), is capable of reacting with short-lived radicals, forming a secondary stable radical, was used for ESR-detection of radical production in isolated cells. The properties of OXANO. and OXANOH in terms of stability in cellular and subcellular systems, membrane permeability and effects on cellular viability were evaluated. Ischemia and reperfusion was simulated in vitro in a preparation of cells from rat intestinal mucosa by incubation at high density (4 x 10^8 cells/ml) under an atmosphere of nitrogen for 25 min and resuspended with fresh oxygenated buffer containing 5 mM Oxanoh. A significant increase in radical formation during the 15 min reperfusion period studied was obtained in cells exposed to ischemia compared to control cells incubated at normal density under an atmosphere of oxygen . The addition of 5 μM of the scavenging enzyme superoxide dismutase reduced the radical formation by 50%. The sequence of the superoxide formation was calculated as the difference in radical production in the presence and absence of superoxide dismutase. |
| hauptsatz | hsatz_simple |
| identnr | NLZ187394903 |
| issn | 0891-5849 |
| journal_name | Free Radical Biology and Medicine |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 6 (1989), S. 251-259 |
| schlagwort | ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase |
| search_space | articles |
| shingle_author_1 | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. |
| shingle_author_2 | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. |
| shingle_author_3 | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. |
| shingle_author_4 | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. |
| shingle_catch_all_1 | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. Detection of oxygen radicals during reperfursion of intestinal cell in vitro ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase 0891-5849 08915849 Elsevier |
| shingle_catch_all_2 | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. Detection of oxygen radicals during reperfursion of intestinal cell in vitro ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase 0891-5849 08915849 Elsevier |
| shingle_catch_all_3 | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. Detection of oxygen radicals during reperfursion of intestinal cell in vitro ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase 0891-5849 08915849 Elsevier |
| shingle_catch_all_4 | Nilsson, U.A. Olsson, L.-I. Thor, H. Moldeus, P. Bylund-Fellenius, A.-C. Detection of oxygen radicals during reperfursion of intestinal cell in vitro ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase ESR Ischemia and reperfusion Nitroxyl radicals Small intestine Spin trapping Superoxide Superoxide dismutase 0891-5849 08915849 Elsevier |
| shingle_title_1 | Detection of oxygen radicals during reperfursion of intestinal cell in vitro |
| shingle_title_2 | Detection of oxygen radicals during reperfursion of intestinal cell in vitro |
| shingle_title_3 | Detection of oxygen radicals during reperfursion of intestinal cell in vitro |
| shingle_title_4 | Detection of oxygen radicals during reperfursion of intestinal cell in vitro |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:49:40.685Z |
| titel | Detection of oxygen radicals during reperfursion of intestinal cell in vitro |
| titel_suche | Detection of oxygen radicals during reperfursion of intestinal cell in vitro The nitroxide OXANO. (2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl) which in its reduced form, OXANOH (2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine), is capable of reacting with short-lived radicals, forming a secondary stable radical, was used for ESR-detection of radical production in isolated cells. The properties of OXANO. and OXANOH in terms of stability in cellular and subcellular systems, membrane permeability and effects on cellular viability were evaluated. Ischemia and reperfusion was simulated in vitro in a preparation of cells from rat intestinal mucosa by incubation at high density (4 x 10^8 cells/ml) under an atmosphere of nitrogen for 25 min and resuspended with fresh oxygenated buffer containing 5 mM Oxanoh. A significant increase in radical formation during the 15 min reperfusion period studied was obtained in cells exposed to ischemia compared to control cells incubated at normal density under an atmosphere of oxygen . The addition of 5 μM of the scavenging enzyme superoxide dismutase reduced the radical formation by 50%. The sequence of the superoxide formation was calculated as the difference in radical production in the presence and absence of superoxide dismutase. |
| topic | W V WW-YZ |
| uid | nat_lic_papers_NLZ187394903 |