A simple phase-extraction assay for chloramphenicol acyltransferase activity
| ISSN: |
0378-1119
|
|---|---|
| Keywords: |
CAT ; cell cultures ; plant cells ; reduced background ; solvent extraction ; transient expression ; tritiated chloramphenicol
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292004314021889 |
|---|---|
| autor | Seed, B. Sheen, J.-Y. |
| autorsonst | Seed, B. Sheen, J.-Y. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0378-1119(88)90403-9 |
| datenlieferant | nat_lic_papers |
| fussnote | A simple and convenient phase extraction assay for chloramphenicol (Cm) acetyltransferase (CAT) activity has been developed, based on the enzymatic butyrylation of radiolabelled Cm. The assay is linear over two to three orders of magnitude of enzyme concentration, is highly sensitive, and substantially less expensive than all presently available alternatives. Methods for convenient CAT assay, adapted for mammalian cells, plant protoplasts, and mammalian cell culture supernatants, are described. These methods should also simplify measurement of CAT activity in other organisms, such as yeast and bacteria. In addition, a simple preextraction procedure is presented for purifying radiolabelled Cm which allows a 25-fold increase in sensitivity using tritiated substrates. |
| hauptsatz | hsatz_simple |
| identnr | NLZ187346267 |
| issn | 0378-1119 |
| journal_name | Gene |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 67 (1988), S. 271-277 |
| schlagwort | CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol |
| search_space | articles |
| shingle_author_1 | Seed, B. Sheen, J.-Y. |
| shingle_author_2 | Seed, B. Sheen, J.-Y. |
| shingle_author_3 | Seed, B. Sheen, J.-Y. |
| shingle_author_4 | Seed, B. Sheen, J.-Y. |
| shingle_catch_all_1 | Seed, B. Sheen, J.-Y. A simple phase-extraction assay for chloramphenicol acyltransferase activity CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol 0378-1119 03781119 Elsevier |
| shingle_catch_all_2 | Seed, B. Sheen, J.-Y. A simple phase-extraction assay for chloramphenicol acyltransferase activity CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol 0378-1119 03781119 Elsevier |
| shingle_catch_all_3 | Seed, B. Sheen, J.-Y. A simple phase-extraction assay for chloramphenicol acyltransferase activity CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol 0378-1119 03781119 Elsevier |
| shingle_catch_all_4 | Seed, B. Sheen, J.-Y. A simple phase-extraction assay for chloramphenicol acyltransferase activity CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol CAT cell cultures plant cells reduced background solvent extraction transient expression tritiated chloramphenicol 0378-1119 03781119 Elsevier |
| shingle_title_1 | A simple phase-extraction assay for chloramphenicol acyltransferase activity |
| shingle_title_2 | A simple phase-extraction assay for chloramphenicol acyltransferase activity |
| shingle_title_3 | A simple phase-extraction assay for chloramphenicol acyltransferase activity |
| shingle_title_4 | A simple phase-extraction assay for chloramphenicol acyltransferase activity |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:41:37.337Z |
| titel | A simple phase-extraction assay for chloramphenicol acyltransferase activity |
| titel_suche | A simple phase-extraction assay for chloramphenicol acyltransferase activity A simple and convenient phase extraction assay for chloramphenicol (Cm) acetyltransferase (CAT) activity has been developed, based on the enzymatic butyrylation of radiolabelled Cm. The assay is linear over two to three orders of magnitude of enzyme concentration, is highly sensitive, and substantially less expensive than all presently available alternatives. Methods for convenient CAT assay, adapted for mammalian cells, plant protoplasts, and mammalian cell culture supernatants, are described. These methods should also simplify measurement of CAT activity in other organisms, such as yeast and bacteria. In addition, a simple preextraction procedure is presented for purifying radiolabelled Cm which allows a 25-fold increase in sensitivity using tritiated substrates. |
| topic | W |
| uid | nat_lic_papers_NLZ187346267 |