Jekyll, a family of phage-plasmid shuttle vectors
| ISSN: |
0378-1119
|
|---|---|
| Keywords: |
Bacteriophage λ ; M13 origin ; polylinker ; recombinant DNA ; subcloning ; vector
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292003985817600 |
|---|---|
| autor | Burmeister, M. Lehrach, H. |
| autorsonst | Burmeister, M. Lehrach, H. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0378-1119(88)90331-9 |
| datenlieferant | nat_lic_papers |
| fussnote | A series of shuttle vectors has been constructed, which consist of a plasmid carrying a polylinker sequence and an M13 origin integrated into a lambda vector. A short direct repeat flanking the plasmid allows plasmid excision by homologous recombination. Sequences are cloned into unique restriction sites within the plasmid, and can be recovered either in phage or plasmid form, or can be packaged further as single-stranded DNA phage. These vectors therefore combine the efficiency of phage λ cloning and screening with the ease of handling or analysing plasmid or M13 clones. |
| hauptsatz | hsatz_simple |
| identnr | NLZ187343659 |
| issn | 0378-1119 |
| journal_name | Gene |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 73 (1988), S. 245-250 |
| schlagwort | Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector |
| search_space | articles |
| shingle_author_1 | Burmeister, M. Lehrach, H. |
| shingle_author_2 | Burmeister, M. Lehrach, H. |
| shingle_author_3 | Burmeister, M. Lehrach, H. |
| shingle_author_4 | Burmeister, M. Lehrach, H. |
| shingle_catch_all_1 | Burmeister, M. Lehrach, H. Jekyll, a family of phage-plasmid shuttle vectors Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector 0378-1119 03781119 Elsevier |
| shingle_catch_all_2 | Burmeister, M. Lehrach, H. Jekyll, a family of phage-plasmid shuttle vectors Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector 0378-1119 03781119 Elsevier |
| shingle_catch_all_3 | Burmeister, M. Lehrach, H. Jekyll, a family of phage-plasmid shuttle vectors Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector 0378-1119 03781119 Elsevier |
| shingle_catch_all_4 | Burmeister, M. Lehrach, H. Jekyll, a family of phage-plasmid shuttle vectors Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector Bacteriophage λ M13 origin polylinker recombinant DNA subcloning vector 0378-1119 03781119 Elsevier |
| shingle_title_1 | Jekyll, a family of phage-plasmid shuttle vectors |
| shingle_title_2 | Jekyll, a family of phage-plasmid shuttle vectors |
| shingle_title_3 | Jekyll, a family of phage-plasmid shuttle vectors |
| shingle_title_4 | Jekyll, a family of phage-plasmid shuttle vectors |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:41:37.337Z |
| titel | Jekyll, a family of phage-plasmid shuttle vectors |
| titel_suche | Jekyll, a family of phage-plasmid shuttle vectors A series of shuttle vectors has been constructed, which consist of a plasmid carrying a polylinker sequence and an M13 origin integrated into a lambda vector. A short direct repeat flanking the plasmid allows plasmid excision by homologous recombination. Sequences are cloned into unique restriction sites within the plasmid, and can be recovered either in phage or plasmid form, or can be packaged further as single-stranded DNA phage. These vectors therefore combine the efficiency of phage λ cloning and screening with the ease of handling or analysing plasmid or M13 clones. |
| topic | W |
| uid | nat_lic_papers_NLZ187343659 |