SOS induction as an in vivo assay of enzyme-DNA interactions

Heitman, J. ; Model, P.

Amsterdam : Elsevier
ISSN:
0378-1119
Keywords:
DNA damage ; DNA double-strand breaks ; DNA metabolism ; DNA methyltransferases ; DNA repair ; DNA-binding proteins ; EcoRI endonuclease ; Escherichia coli ; SOS response ; methylation-dependent restriction ; restriction-modification
Source:
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
Topics:
Biology
Type of Medium:
Electronic Resource
URL:
_version_ 1798292001238548480
autor Heitman, J.
Model, P.
Heitman, J.
Model, P.
autorsonst Heitman, J.
Model, P.
Heitman, J.
Model, P.
book_url http://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90383-M
datenlieferant nat_lic_papers
fussnote We have constructed strains which are convenient and sensitive indicators of DNA damage and describe their use. These strains utilize an SOS ::lacZ fusion constructed by Kenyon and Walker [Proc. Natl. Acad. Sci. USA 77 (1980) 2819-2823] and respond to DNA damage by producing β-galactosidase. They can be used to characterize restriction systems and screen for restriction endonuclease mutants. Applications include the study of other enzymes involved in DNA metabolism, such as DNA methyltransferases, topoisomerases, recombinases, and DNA replication and repair enzymes.
hauptsatz hsatz_simple
identnr NLZ187315612
issn 0378-1119
journal_name Gene
materialart 1
package_name Elsevier
publikationsort Amsterdam
publisher Elsevier
reference 103 (1991), S. 1-9
schlagwort DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
search_space articles
shingle_author_1 Heitman, J.
Model, P.
Heitman, J.
Model, P.
shingle_author_2 Heitman, J.
Model, P.
Heitman, J.
Model, P.
shingle_author_3 Heitman, J.
Model, P.
Heitman, J.
Model, P.
shingle_author_4 Heitman, J.
Model, P.
Heitman, J.
Model, P.
shingle_catch_all_1 Heitman, J.
Model, P.
Heitman, J.
Model, P.
SOS induction as an in vivo assay of enzyme-DNA interactions
DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
0378-1119
03781119
Elsevier
shingle_catch_all_2 Heitman, J.
Model, P.
Heitman, J.
Model, P.
SOS induction as an in vivo assay of enzyme-DNA interactions
DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
0378-1119
03781119
Elsevier
shingle_catch_all_3 Heitman, J.
Model, P.
Heitman, J.
Model, P.
SOS induction as an in vivo assay of enzyme-DNA interactions
DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
0378-1119
03781119
Elsevier
shingle_catch_all_4 Heitman, J.
Model, P.
Heitman, J.
Model, P.
SOS induction as an in vivo assay of enzyme-DNA interactions
DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
DNA damage
DNA double-strand breaks
DNA metabolism
DNA methyltransferases
DNA repair
DNA-binding proteins
EcoRI endonuclease
Escherichia coli
SOS response
methylation-dependent restriction
restriction-modification
0378-1119
03781119
Elsevier
shingle_title_1 SOS induction as an in vivo assay of enzyme-DNA interactions
shingle_title_2 SOS induction as an in vivo assay of enzyme-DNA interactions
shingle_title_3 SOS induction as an in vivo assay of enzyme-DNA interactions
shingle_title_4 SOS induction as an in vivo assay of enzyme-DNA interactions
sigel_instance_filter dkfz
geomar
wilbert
ipn
albert
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source_archive Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
timestamp 2024-05-06T08:41:34.774Z
titel SOS induction as an in vivo assay of enzyme-DNA interactions
titel_suche SOS induction as an in vivo assay of enzyme-DNA interactions
We have constructed strains which are convenient and sensitive indicators of DNA damage and describe their use. These strains utilize an SOS ::lacZ fusion constructed by Kenyon and Walker [Proc. Natl. Acad. Sci. USA 77 (1980) 2819-2823] and respond to DNA damage by producing β-galactosidase. They can be used to characterize restriction systems and screen for restriction endonuclease mutants. Applications include the study of other enzymes involved in DNA metabolism, such as DNA methyltransferases, topoisomerases, recombinases, and DNA replication and repair enzymes.
topic W
uid nat_lic_papers_NLZ187315612