SOS induction as an in vivo assay of enzyme-DNA interactions
| ISSN: |
0378-1119
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|---|---|
| Keywords: |
DNA damage ; DNA double-strand breaks ; DNA metabolism ; DNA methyltransferases ; DNA repair ; DNA-binding proteins ; EcoRI endonuclease ; Escherichia coli ; SOS response ; methylation-dependent restriction ; restriction-modification
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292001238548480 |
|---|---|
| autor | Heitman, J. Model, P. Heitman, J. Model, P. |
| autorsonst | Heitman, J. Model, P. Heitman, J. Model, P. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90383-M |
| datenlieferant | nat_lic_papers |
| fussnote | We have constructed strains which are convenient and sensitive indicators of DNA damage and describe their use. These strains utilize an SOS ::lacZ fusion constructed by Kenyon and Walker [Proc. Natl. Acad. Sci. USA 77 (1980) 2819-2823] and respond to DNA damage by producing β-galactosidase. They can be used to characterize restriction systems and screen for restriction endonuclease mutants. Applications include the study of other enzymes involved in DNA metabolism, such as DNA methyltransferases, topoisomerases, recombinases, and DNA replication and repair enzymes. |
| hauptsatz | hsatz_simple |
| identnr | NLZ187315612 |
| issn | 0378-1119 |
| journal_name | Gene |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 103 (1991), S. 1-9 |
| schlagwort | DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification |
| search_space | articles |
| shingle_author_1 | Heitman, J. Model, P. Heitman, J. Model, P. |
| shingle_author_2 | Heitman, J. Model, P. Heitman, J. Model, P. |
| shingle_author_3 | Heitman, J. Model, P. Heitman, J. Model, P. |
| shingle_author_4 | Heitman, J. Model, P. Heitman, J. Model, P. |
| shingle_catch_all_1 | Heitman, J. Model, P. Heitman, J. Model, P. SOS induction as an in vivo assay of enzyme-DNA interactions DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification 0378-1119 03781119 Elsevier |
| shingle_catch_all_2 | Heitman, J. Model, P. Heitman, J. Model, P. SOS induction as an in vivo assay of enzyme-DNA interactions DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification 0378-1119 03781119 Elsevier |
| shingle_catch_all_3 | Heitman, J. Model, P. Heitman, J. Model, P. SOS induction as an in vivo assay of enzyme-DNA interactions DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification 0378-1119 03781119 Elsevier |
| shingle_catch_all_4 | Heitman, J. Model, P. Heitman, J. Model, P. SOS induction as an in vivo assay of enzyme-DNA interactions DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification DNA damage DNA double-strand breaks DNA metabolism DNA methyltransferases DNA repair DNA-binding proteins EcoRI endonuclease Escherichia coli SOS response methylation-dependent restriction restriction-modification 0378-1119 03781119 Elsevier |
| shingle_title_1 | SOS induction as an in vivo assay of enzyme-DNA interactions |
| shingle_title_2 | SOS induction as an in vivo assay of enzyme-DNA interactions |
| shingle_title_3 | SOS induction as an in vivo assay of enzyme-DNA interactions |
| shingle_title_4 | SOS induction as an in vivo assay of enzyme-DNA interactions |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:41:34.774Z |
| titel | SOS induction as an in vivo assay of enzyme-DNA interactions |
| titel_suche | SOS induction as an in vivo assay of enzyme-DNA interactions We have constructed strains which are convenient and sensitive indicators of DNA damage and describe their use. These strains utilize an SOS ::lacZ fusion constructed by Kenyon and Walker [Proc. Natl. Acad. Sci. USA 77 (1980) 2819-2823] and respond to DNA damage by producing β-galactosidase. They can be used to characterize restriction systems and screen for restriction endonuclease mutants. Applications include the study of other enzymes involved in DNA metabolism, such as DNA methyltransferases, topoisomerases, recombinases, and DNA replication and repair enzymes. |
| topic | W |
| uid | nat_lic_papers_NLZ187315612 |