Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp.
| ISSN: |
0378-1119
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|---|---|
| Keywords: |
Cyanobacteria ; gene organization ; gene sequences ; phylogeny ; protein structure ; subunits ; thylakakoid lumen ; transcripts
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798291999463309312 |
|---|---|
| autor | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. |
| autorsonst | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0378-1119(93)90618-D |
| datenlieferant | nat_lic_papers |
| fussnote | We have isolated the genes encoding 11 photosystem I (PSI) subunits from Synechococcus sp., from which this reaction center has been crystallized. The recombinant DNAs, including psaA, psaB, psaC, psaD, psaE, psaF, psal, psaJ, psaK and psaL, were obtained by heterologous hybridization with probes from appropriate cDNAs or genes from spinach and Synechocystis sp. PCC 6803, or with synthetic oligodeoxyribonucleotides. Genes psaA/psaB, psaF/psaJ and psaL/psal are each closely linked. The open reading frames predict polypeptides of 83 kDa (subunits la and Ib, encoded by genes psaA and psaB, respectively), 15.4 kDa (II, psaD), 17.7 kDa (III, psaF), 8.4 kDa (IV, psaE), 8.8 kDa (VII, psaC), 4.6 kDa (VIII, psal), 4.8 kDa (IX, psaJ), 8.5 kDa (X, psaK) and 15.5 kDa (XI, psaL). A novel subunit (XII, psaM) was also identified. Subunits II, III, IV and VII seem to be peripheral, while the others seem to be intrinsic components of the reaction center. These data imply a striking similarity of cyanobacterial and eukaryotic PSI. All subunits studied are encoded by single-copy genes which seem to be transcribed into monocistronic (psaC, psaD, psaC, psaK) or dicistronic (psaA/psaB, psaF/psaJ, psaL/psal) RNA species. Subunit III is translated as a 17.7-kDa precursor, including a transit peptide of 23 amino acid residues. This is consistent with its location in the thylakoid lumen. |
| hauptsatz | hsatz_simple |
| identnr | NLZ187301816 |
| issn | 0378-1119 |
| journal_name | Gene |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 127 (1993), S. 71-78 |
| schlagwort | Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts |
| search_space | articles |
| shingle_author_1 | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. |
| shingle_author_2 | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. |
| shingle_author_3 | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. |
| shingle_author_4 | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. |
| shingle_catch_all_1 | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts 0378-1119 03781119 Elsevier |
| shingle_catch_all_2 | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts 0378-1119 03781119 Elsevier |
| shingle_catch_all_3 | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts 0378-1119 03781119 Elsevier |
| shingle_catch_all_4 | Muhlenhoff, U. Haehnel, W. Witt, H. Herrmann, R.G. Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts Cyanobacteria gene organization gene sequences phylogeny protein structure subunits thylakakoid lumen transcripts 0378-1119 03781119 Elsevier |
| shingle_title_1 | Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. |
| shingle_title_2 | Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. |
| shingle_title_3 | Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. |
| shingle_title_4 | Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:41:33.005Z |
| titel | Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. |
| titel_suche | Genes encoding eleven subunits of photosystem I from the thermophilic cyanobacterium Synechococcus sp. We have isolated the genes encoding 11 photosystem I (PSI) subunits from Synechococcus sp., from which this reaction center has been crystallized. The recombinant DNAs, including psaA, psaB, psaC, psaD, psaE, psaF, psal, psaJ, psaK and psaL, were obtained by heterologous hybridization with probes from appropriate cDNAs or genes from spinach and Synechocystis sp. PCC 6803, or with synthetic oligodeoxyribonucleotides. Genes psaA/psaB, psaF/psaJ and psaL/psal are each closely linked. The open reading frames predict polypeptides of 83 kDa (subunits la and Ib, encoded by genes psaA and psaB, respectively), 15.4 kDa (II, psaD), 17.7 kDa (III, psaF), 8.4 kDa (IV, psaE), 8.8 kDa (VII, psaC), 4.6 kDa (VIII, psal), 4.8 kDa (IX, psaJ), 8.5 kDa (X, psaK) and 15.5 kDa (XI, psaL). A novel subunit (XII, psaM) was also identified. Subunits II, III, IV and VII seem to be peripheral, while the others seem to be intrinsic components of the reaction center. These data imply a striking similarity of cyanobacterial and eukaryotic PSI. All subunits studied are encoded by single-copy genes which seem to be transcribed into monocistronic (psaC, psaD, psaC, psaK) or dicistronic (psaA/psaB, psaF/psaJ, psaL/psal) RNA species. Subunit III is translated as a 17.7-kDa precursor, including a transit peptide of 23 amino acid residues. This is consistent with its location in the thylakoid lumen. |
| topic | W |
| uid | nat_lic_papers_NLZ187301816 |