Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer
| ISSN: |
0167-4838
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|---|---|
| Keywords: |
CNBr fraction ; Cartilage ; N-terminal sequence ; Proteoglycan
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| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Chemistry and Pharmacology
Medicine
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798291644366192641 |
|---|---|
| autor | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. |
| autorsonst | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0167-4838(83)90420-X |
| datenlieferant | nat_lic_papers |
| fussnote | Cyanogen bromide treatment of bovine nasal cartilage proteoglycan monomer gave rise to three major fractions (CN-1 to CN-3), isolated by Sepharose CL-6B chromatography. The uronate-rich fraction in the void volume (CN-1) digested with chondroitinase ABC (C treatment) yielded a fragment (CN-1 C/6B) with a unique N-terminal sequence. The same fraction, when digested sequentially by chondroitinase ABC and trypsin (CT treatment), was resolved into two distinct fractions, CN-1 CT/6B-1 and CN-1 CT/6B-2. CN-1 CT/6B-1 consisted in a keratan sulfate-rich region, representing the N-terminal moiety of the CN-1 fraction; these data suggested, according to the model of the proteoglycan monomer structure described by Heinegard, D. and Axelsson, I. (1977) J. Biol. Chem. 252, 1971-1979, that its C-terminal moiety is localized at the end of the core bearing the chondroitin sulfate chains. CN-1 CT/6B-2 contained two fragments from the chondroitin sulfate-bearing region: one of them has been submitted to Edman degradation. The CN-2 fraction upon cbondroitinase and trypsin treatments gave rise to a keratan-bearing region (CN-2 CT/6B-1) and a mannose-rich region (CN-2 CT/6B-2). After reduction and alkylation of CN-2, the N-terminal sequence of the isolated major fragment (CN-2 RA/6B-1) was determined. The CN-3 fraction revealed a pattern upon electrophoresis similar to that of the cyanogen bromide-treated hyalurouic acid-binding region. |
| hauptsatz | hsatz_simple |
| identnr | NLZ18690505X |
| issn | 0167-4838 |
| journal_name | Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 743 (1983), S. 82-90 |
| schlagwort | CNBr fraction Cartilage N-terminal sequence Proteoglycan |
| search_space | articles |
| shingle_author_1 | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. |
| shingle_author_2 | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. |
| shingle_author_3 | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. |
| shingle_author_4 | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. |
| shingle_catch_all_1 | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer CNBr fraction Cartilage N-terminal sequence Proteoglycan CNBr fraction Cartilage N-terminal sequence Proteoglycan 0167-4838 01674838 Elsevier |
| shingle_catch_all_2 | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer CNBr fraction Cartilage N-terminal sequence Proteoglycan CNBr fraction Cartilage N-terminal sequence Proteoglycan 0167-4838 01674838 Elsevier |
| shingle_catch_all_3 | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer CNBr fraction Cartilage N-terminal sequence Proteoglycan CNBr fraction Cartilage N-terminal sequence Proteoglycan 0167-4838 01674838 Elsevier |
| shingle_catch_all_4 | Bonnet, F. Le Gledic, S. Perin, J.-P. Jolles, J. Jolles, P. Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer CNBr fraction Cartilage N-terminal sequence Proteoglycan CNBr fraction Cartilage N-terminal sequence Proteoglycan 0167-4838 01674838 Elsevier |
| shingle_title_1 | Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer |
| shingle_title_2 | Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer |
| shingle_title_3 | Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer |
| shingle_title_4 | Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:35:52.393Z |
| titel | Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer |
| titel_suche | Identification of cyanogen bromide-fragments of the protein core of bovine nasal cartilage proteoglycan monomer Cyanogen bromide treatment of bovine nasal cartilage proteoglycan monomer gave rise to three major fractions (CN-1 to CN-3), isolated by Sepharose CL-6B chromatography. The uronate-rich fraction in the void volume (CN-1) digested with chondroitinase ABC (C treatment) yielded a fragment (CN-1 C/6B) with a unique N-terminal sequence. The same fraction, when digested sequentially by chondroitinase ABC and trypsin (CT treatment), was resolved into two distinct fractions, CN-1 CT/6B-1 and CN-1 CT/6B-2. CN-1 CT/6B-1 consisted in a keratan sulfate-rich region, representing the N-terminal moiety of the CN-1 fraction; these data suggested, according to the model of the proteoglycan monomer structure described by Heinegard, D. and Axelsson, I. (1977) J. Biol. Chem. 252, 1971-1979, that its C-terminal moiety is localized at the end of the core bearing the chondroitin sulfate chains. CN-1 CT/6B-2 contained two fragments from the chondroitin sulfate-bearing region: one of them has been submitted to Edman degradation. The CN-2 fraction upon cbondroitinase and trypsin treatments gave rise to a keratan-bearing region (CN-2 CT/6B-1) and a mannose-rich region (CN-2 CT/6B-2). After reduction and alkylation of CN-2, the N-terminal sequence of the isolated major fragment (CN-2 RA/6B-1) was determined. The CN-3 fraction revealed a pattern upon electrophoresis similar to that of the cyanogen bromide-treated hyalurouic acid-binding region. |
| topic | W V WW-YZ |
| uid | nat_lic_papers_NLZ18690505X |